Vector Core

核心A-向量核心 宾夕法尼亚大学的向量核心实验室是一个独立的全方位服务实验室，提供 基因转移领域的试剂和专业知识。这个名为“宾夕法尼亚矢量”的机构是由朱莉博士指导的 约翰斯顿在AAV、腺病毒和慢病毒载体生产领域管理着8名科学家。.这就是 该计划将重点利用宾夕法尼亚大学的载体来生产AAV载体，这些载体将服务于项目I 由Pranay Khare博士指导的独立质量控制程序执行了一系列 用于体内研究的载体释放所需的分析。这个核心还将分析组织中的存在 利用TaqMan和聚合酶链式反应技术对载体基因组进行分析。最后，向量核心将适应现有的方法 制造和提纯到临床候选的大规模生产的效率足以 支持大型动物研究。实验室在普洛斯的一般原则下运作，包括： 文件(批次记录表)、SOP、QA/QC、培训和设备验证，尽管不是 表示自己是完全的GLP或GMP。 版面描述。P01中使用的所有矢量都将由矢量核心创建和生成。 相关性(请参阅说明)：

Core A- Vector Core The Vector Core at the University of Pennsylvania is an independent and full service laboratory providing reagents and expertise in the area of gene transfer. This facility called "Penn Vector" is directed by Dr. Julie Johnston who manages 8 scientists in the area of AAV, adenoviral and lentiviral vector production. .This program will focus on utilization of Penn vector for the production of AAV vectors that will service Projects I through III. An independent Quality Control Program directed by Dr. Pranay Khare performs a series of assays necessary for release of the vector for in vivo studies. This core will also analyze tissues for presence of vector genomes using TaqMan and PCR technology. Finally, the Vector Core will adapt existing methods of manufacturing and purification to the clinical candidate for a large scale production efficient enough to support large animal studies. The laboratory operates under the general principles of GLP including, documentation (batch record forms), SOPs, QA/QC, training and equipment validation, although it does not represent itself as being fully GLP or GMP. Lay description. All vectors used in the P01 will be created and produced by the Vector Core. RELEVANCE (Seeinstructions):