INBRE: URI: CENTRALIZED RES CORE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 国际扶轮-INBRE中央研究核心基金向网络参与者和罗德岛生物医学研究社区提供研究和培训支持已进入第六个年头。在本赠款期间，超过55个研究小组使用了该机制，平均季度累计使用频率为1 012次。该机构的用户包括30名教职员工、23名博士后研究员、65名研究生和35名本科生。在过去一年中，中央研究核心设施的使用产生了一份发表的摘要和22份承认使用该设施的出版物。该设施的工作人员向该设施的所有新用户提供培训，并就在其研究项目中使用各种技术提供咨询。此外，工作人员还代表该机构参加了在佛蒙特州伯灵顿举行的东北地区生命科学核心董事会议和在田纳西州孟菲斯举行的生物分子资源设施协会会议。他们还继续参与在药学院提供的本科生和研究生实验室课程中教授各种技术。这些课程旨在为学生提供使用该设施设备的理论和实践经验。在这一年中，与仪器制造商合作举办了几次教学研讨会和培训讲习班。主题包括：数字蛋白质芯片，一种用于质谱仪样品制备和分析的技术；ECL-Plex多重荧光定量Western blotting；以及闪光色谱技术的进展。今年采购的新设备包括GloMax-96双注射器微板发光仪、CombiFlash色谱系统和带有毛细管池的Nano DSC系统，以及用于流动细胞测量数据分析的Fas-3软件。此外，还对DNA阵列系统和Flow细胞测量系统进行了升级。由于参与机构继续为维持该机制提供资金，因此向这些机构的研究人员免费提供设备和资源。总共印制了119张海报，非国际扶轮-INBRE参与者支付了适度的费用来支付消耗品的费用。在未来，计划举办以下研讨会：生物标记物的发现，用于蛋白质、DNA和RNA分析的LabChip系统，以及用于细胞分析的xCELLigence系统。我们通过电子邮件通信和在2009年1月重试的冬季进行的调查，收集了国际扶轮INBRE调查人员对未来购买的工具建议。总体而言，核心设施工具及其咨询服务的使用继续增加。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The RI-INBRE Centralized Research Core Facility is in its sixth year of providing research and training support to the Network participants and the Rhode Island biomedical research community. During the current grant period more than 55 research groups used the Facility, with an average quarterly cumulative frequency of 1,012 usages. The Facility users consisted of 30 faculty, 23 postdoctoral fellows, 65 graduate students, and 35 undergraduate students. Usage of the Centralized Research Core Facility has resulted in one published abstract and twenty-two publications acknowledging use of the facility over the past year. The Facility staff provided training to all new users of the facility and provided consultations for the use of various technologies in their research projects. In addition, the staff represented the Facility at the Northeast Regional Life Sciences Core Directors Meeting in Burlington, Vermont, and the Association of Biomolecular Resource Facility Meeting in Memphis, Tennessee. They also continued to participate in teaching various techniques in the undergraduate and graduate laboratory courses offered by the College of Pharmacy. These courses are designed to provide both theoretical and hands-on experience to students in using the Facility's equipment. Throughout the year, several instructional seminars and training workshops were organized in collaboration with the instrument manufacturers. The topics included: The Digital ProteomeChip, a Technology for Mass Spec Sample Preparation and Analysis; ECL-Plex Multiplex Fluorescent Quantitative Western Blotting; and Advances in Flash Chromatography Technique. New equipment acquired this year included a GloMax-96 microplate luminometer with dual injectors, a CombiFlash chromatography system and Nano DSC system with capillary cell, and FAS-3 software for flow cytometery data analysis. Also, the DNA array system and the Flow cytometery system were upgrade. Since the participating institutions continue to contribute funds towards maintaining the Facility, the equipment and resources were made available to researchers from these institutions at no cost. A total of 119 posters were printed with non-RI-INBRE participants paying a modest fee to cover the cost of consumables. In the future, seminars on the following: Biomarkers discovery, The LabChip system for protein, DNA and RNA analysis, and xCELLigence system for cellular analysis are planned. We collected instrument recommendations from RI-INBRE investigators for future purchases via e-mail communications and also through the survey conducted at the winter retried in January 2009. Overall, the usage of the Core Facility instrumentation and its consultative services continues to increase.