Electron Spin Resonance Core

核心领导人Dikalov博士是血管细胞和组织中ROS和NO体外和离体测量的专家，并一直处于开发使用ESR光谱进行ROS检测的新方法的最前沿。他的大部分工作都集中在开发新的ESR技术，用于ROS的体外和体内测量（12 - 14）。虽然ESR光谱可以直接检测自由基产物，但大多数 生物学相关的自由基的寿命太短而不能在生物样品中检测到。为此，已经使用自旋捕获和自旋探针。自旋捕集已被证明是用于ROS检测和定量的最确定的方法之一。CORE A已经广泛发表了关于使用硝酮自旋阱和Fe（DETC）2的O2~和NO的自旋捕获的文章（15 - 17）。此外，俄罗斯新西伯利亚有机化学研究所的Dikalov博士和同事设计并验证了多种新型环状羟胺 其作为自旋探针，为ROS检测提供增强的灵敏度（14，18）。 核心A的重点是培养细胞和完整组织样本中的ROS和NO。Dikalov博士还开发了一种方法，使用过氧化物酶介导的CPH氧化来测量膜组分中过氧化氢（H2O2）的产生。此外，电子自旋共振核心将开发新的方法，并完善现有的技术，用于测量相关组织中的ROS。这些方法的应用将提高个别项目领导人的能力，以检验他们的假设和设计新的 必要的研究，以促进他们的工作。

The core leader, Dr. Dikalov, is an expert in in vitro and ex vivo measurements of ROS and NO in vascular cells and tissues and has been at the forefront in developing new methodologies for ROS detection using ESR spectroscopy. Much of his work has been focused on development of new ESR techniques for in vitro and in vivo measurements of ROS (12-14). While ESR spectroscopy can directly detect free radical products, most biologically relevant radicals are far too short-lived to be detected in biological specimens. For this reason, spin-trapping and spin probes have been used. Spin trapping has proven to be one of the most definitive^ methods for ROS detection and quantification. CORE A has published extensively on spin trapping of O2~ and NO using nitrone spin traps and Fe(DETC)2 (15-17). Moreover, Dr. Dikalov and colleagues at the Institute of Organic Chemistry in Novosibirsk, Russia have designed and validated a variety of new cyclic hydroxylamines which act as spin probes that provide enhanced sensitivity for ROS detection (14, 18). The focus of Core A will be the ROS and NO in cultured cells and intact tissue samples. Dr. Dikalov has also developed an approach to measure the production of hydrogen peroxide (H2O2) in membrane fractions using a peroxidase-mediated oxidation of CPH. In addition, the Electron Spin Resonance Core will develop new methods and refine existing techniques for measurements of ROS in relevant tissues. The application of these methods will enhance the ability of the individual project leaders to test their hypotheses and design new studies as necessary to advance their work.