MAINTENANCE AND REPAIR OF THE IVEM F20

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本报告所述期间（2008年6月1日至2009年5月31日）。显微镜被广泛使用（大约。20个规则 用户）。我们总共获得了269个倾斜系列（88个低温和182个室温）。我们几乎没有 在显微镜上完成的维修工作（约16天）。显微镜在典型的 工作日（8-6 M-F），82%的工作在这些时间内完成。此外，显微镜是 去年，我使用了360天中的285天，平均每周38.5小时（2006小时/52 周）。F20用于冷冻成像的占48%，室温成像的占24%， 冷冻断层扫描，7%用于服务，5%用于培训，1%用于开发。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this reporting period (6/1/08-5/31/09). The microscope was used extensively (approx. 20 regular users). We acquired a total of 269 tilt-series (88 cryo and 182 room temperature). We had very little service work (~16 days) done on the microscope. The microscope was heavily used during the typical workday (8-6 M-F) with 82% of our work done during those hours. In addition, the microscope was used 285 of the 360 available days last year with an average of 38.5 hours/week (2006 hours/52 weeks). The F20 was used 48% for cryo-imaging, 24% room temperature imaging, 9% for cryo-tomography, 7% for service, 5% for training, and 1% for development.