GENE EXPRESSION NERVOUS SYSTEM ATLAS (GENSAT)

该合同的总体目标是1)继续对发育过程中小鼠神经系统的基因表达模式进行高通量分析；2)为科学界产生新的小鼠基因研究工具。使用两种互补和协调的技术(标准放射原位杂交和BAC转基因报告鼠)，合同在相对有限的神经系统部分(10到20个)上筛选大量(例如，数千)基因产物的探针。NINDS选择的切面包括神经科学界最感兴趣的结构(例如，新皮质、基底节、海马体、小脑、脊髓等)。在发育的3个或4个阶段(胚胎早期、胚胎晚期和出生后早期)和成年小鼠中分析了基因表达的空间位置/模式。基因表达的结果图像被数字化并发布在网络可访问的数据库中，根据合同产生的所有BAC转基因小鼠都保存在NIH赞助的小鼠储存库中。

The overall objective of this contract is to 1) continue the high-throughput analysis of gene expression patterns in the mouse nervous system during development; 2) generate new mouse genetic research tools for the scientific community. Using two complementary and coordinated technologies (standard radiometric in situ hybridization and BAC transgenic reporter mice), the contract screens probes for a large number (e.g., thousands) of gene products on a relatively limited number (10 to 20) of sections of the nervous system. Planes of section are chosen by the NINDS to include the structures of most general interest to the neuroscience community (e.g., neocortex, basal ganglia, hippocampus, cerebellum, spinal cord, etc.). The spatial locations/patterns of gene expression are analyzed at 3 or 4 stages of development (early and late embryonic, and early postnatal) and in adult mice. The resulting images of gene expression are digitized and posted in Web-accessible databases, and all BAC transgenic mice generated by the contract are deposited in an NIH-sponsored mouse repository.