The Oral Microbiome and Upper Aerodigestive Squamous Cell Cancer

描述(申请人提供)：口腔微生物组和上空气消化鳞状细胞癌我们建议进行一项创新研究，以检查口腔微生物组是否与上空气消化系鳞状细胞癌(UADSCC：口腔、咽、喉和食道鳞状细胞癌)的风险有关。我们的研究团队已经开发出了进行微生物组研究的独特能力(NIH拨款：1UH2 CA140233，5R01AI063477)，最近报告了前肠微生物组与食道癌前疾病的关系(Yang等人，胃肠病学，2009)，现在有初步数据显示口腔微生物组与口腔癌风险之间的关联。烟草和酒精是UADSCC的主要已知危险因素。我们假设口腔中的微生物增强了UADSC的致癌作用，这可能与酒精和烟草的使用有关。我们研究的目标是在这一主题的首次流行病学调查中，将口腔微生物组与UADSCC风险联系起来，并确定酒精和烟草使用对口腔微生物组的影响。我们计划通过对口腔洗液样本中的16S rRNA微生物基因进行测序，来表征140例UADSCC病例和420名对照中所有常见的口腔微生物种类(包括非培养物)。由于UADSCC疾病和治疗状态会改变口腔微生物谱(反向原因)，我们在ACS和PLCO队列中使用前瞻性研究设计，在疾病发展之前收集口腔洗涤样本。我们的研究将确定与UADSCC风险相关的口腔细菌谱，为实施预防干预措施提供直接线索，作为UADSCC预防中酒精和烟草控制计划的关键辅助措施。 公共卫生相关性：拟议的项目是一项创新研究，旨在研究口腔微生物群是否与上呼吸道消化细胞癌(UADSCC：口腔、咽、喉和食道鳞状细胞癌)的风险有关。我们研究的目标是在这一主题的首次流行病学调查中，将口腔微生物组与UADSCC风险联系起来，并确定酒精和烟草使用对口腔微生物组的影响。

DESCRIPTION (provided by applicant): The Oral Microbiome and Upper Aerodigestive Squamous Cell Cancer We propose an innovative study to examine whether the oral microbiome is associated with risk of upper aerodigestive squamous cell cancers (UADSCC: oral, pharyngeal, laryngeal and esophageal squamous cell cancers). Our research team has developed a unique capability to conduct microbiome research (NIH grant: 1UH2CA140233, 5R01AI063477), have recently reported the relationship of foregut microbiome profiles with esophageal premalignant conditions (Yang et al., Gastroenterology, 2009), and now have preliminary data showing an association between the oral microbiome and oral cancer risk. Tobacco and alcohol are the major known risk factors for UADSCC. We hypothesize that microorganisms in the oral cavity potentiate UADSC carcinogenesis, potentially related to alcohol and tobacco use. The goals of our study are to relate the oral microbiome to UADSCC risk and to identify the impact of alcohol and tobacco use on the oral microbiome, in the first epidemiologic investigation of this topic. We plan to characterize, in 140 UADSCC cases and 420 controls, all common oral microbial species (including non-culturables) by sequencing the 16S rRNA microbial genes in oral wash samples. Because UADSCC disease and treatment status can alter oral microbial profiles (reverse causation), we are using a prospective research design, in the ACS and PLCO cohorts in which oral wash samples were collected prior to disease development. Our study will identifying oral bacterial profiles related to UADSCC risk, providing direct leads to implement prophylactic interventions as critical adjuncts to alcohol and tobacco control programs in UADSCC prevention. PUBLIC HEALTH RELEVANCE: The proposed project is an innovative study to examine whether the oral microbiome is associated with risk of upper aerodigestive cell cancers (UADSCC: oral, pharyngeal, laryngeal and esophageal squamous cell cancers). The goals of our study are to relate the oral microbiome to UADSCC risk and to identify the impact of alcohol and tobacco use on the oral microbiome, in the first epidemiologic investigation of this topic.