DIETARY FRUCTOSE AND URINARY OXALATE EXCRETION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 肾结石是一种常见的医学疾病，在其一生中的某个时候折磨10%的白人男性，其中80%的结石主要是草酸钙。尿液中排出的草酸盐量是结石形成的重要危险因素，并且来自饮食摄入和内源性合成。草酸盐的内源性合成由糖和氨基酸的代谢产生，并且主要发生在肝脏中。最近，果糖的消耗被确定为结石形成的危险因素。受试者被分为五分之一，并获得5.6%至15.2%的能量作为果糖。几条证据表明，果糖代谢比葡萄糖代谢导致更多的草酸合成。Rofe等报道，在离体大鼠肝细胞中，糖浓度为10 mM时，果糖产生的草酸盐是葡萄糖的10倍。我们最近在未发表的初步实验中观察到，培养的人肝细胞在含有果糖的培养基中生长时，合成的草酸盐是含有葡萄糖的培养基的3倍。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Kidney stone disease is a prevalent medical condition that afflicts 10% of Caucasian males at some point in their lifetime with 80% of these stones being predominately calcium oxalate. The amount of oxalate excreted in urine is an important risk factor for stone formation and is derived about equally from dietary intake and endogenous synthesis. The endogenous synthesis of oxalate results from the metabolism of sugars and amino acids and occurs primarily in the liver. Fructose consumption was recently identified as a risk factor for stone formation. Subjects were divided into quintiles and received between 5.6% and 15.2% of their energy as fructose. Several lines of evidence indicate that fructose metabolism results in greater synthesis of oxalate than from the metabolism of glucose. With isolated rat hepatocytes, Rofe et el reported that at sugar concentrations of 10mM, fructose produced 10 times more oxalate than glucose. We have recently observed in preliminary, unpublished experiments that cultured human hepatocytes synthesize 3 times as much oxalate when growing in media containing fructose compared with media containing glucose.