Membrane Protein Polyhedral Nanoparticles for High Resolution Structure/Function Studies

 描述(申请人提供)：这项建议旨在使用一种新的方法来研究膜蛋白的结构和功能，该方法将微流控膜自组装方法与高通量电子显微镜单颗粒分析相结合。我们将利用我们以前的工作来实现一种系统的方法来分析生物膜中的膜蛋白结构。这项提议的总体目标将是在没有和存在改变膜蛋白活性的配体和跨膜梯度的情况下确定几种膜蛋白的结构。0925-0001/0002(08/12版)页面续格式页面

 DESCRIPTION (provided by applicant): This proposal aims to study the structure and function of membrane proteins using a novel approach that combines a microfluidic membrane self-assembling method with high throughput electron microscopy single particle analysis. We will leverage our previous work to realize a systematic approach to structural analysis of membrane proteins in a biological membrane. The overall objective of this proposal will be determining the structures of several membrane proteins both in the absence and presence of ligands and transmembrane gradients that alter the activity of the membrane protein. 0925-0001/0002 (Rev. 08/12) Page Continuation Format Page