Shared Resource: Pharmacology

摘要 药理学核心的使命是提供最先进的生物分析技术和广泛的 能够评估临床试验中的关键药理学终点的药理学专业知识 临床前研究。药理学核心被归入交叉学科研究核心群 除药理学核心外，还包括生物统计学、基因组学和生物库 和相关科学核心。 在收费的基础上提供两项服务，即生物检疫处理和生物分析。生物胺 处理是获取、处理和运送患者样本的集中资源 (包括血液和骨髓样本)，以评估药物动力学或 根据临床方案规范进行药效学研究，以促进和支持临床和实验室 研究。生物分析提供高性能液体的开发、验证和实施 基于高效液相色谱法的分析方法，用于药物、代谢物或 生物样品(包括生物流体、组织和细胞培养样品)中的内源性化合物。此外, 提供广泛的药理学支持，包括：1)药代动力学研究设计；2) 使用传统的隔室和非隔室分析进行药代动力学数据分析和建模， 非线性混合效应(群体)药代动力学模型和基于生理的药代动力学 建模；3)体外药物代谢研究；4)代谢物鉴定；5)药物血浆测定 蛋白质结合和血浆与血液的比率。 核心配备了最先进的分析仪器(如AB SCIEX QTRAP 6500 LC- MS/MS系统)和药代动力学分析软件。实验室地处中心位置，交通便利 所有KCI调查员都可以进入。 年期间，药理学核心提供的服务为92份同行评议出版物作出了贡献 当前审核期。

ABSTRACT The Pharmacology Core's mission is to provide state-of-the-art bioanalytic technology and a broad range of pharmacology expertise to enable evaluation of critical pharmacological endpoints in clinical trials and preclinical studies. The Pharmacology Core is grouped in the Cross Disciplinary Research Core Cluster which, in addition to the Pharmacology Core, includes the Biostatistics, Genomics, and the Biobanking and Correlative Sciences Cores. Two services, Biospecimen Processing and Bioanalysis, are provided on a fee-for-service basis. Biospecimen Processing is a centralized resource for the acquisition, processing, and shipment of patient specimens (including blood and bone marrow samples) that are required for evaluation of pharmacokinetics or pharmacodynamics according to clinical protocol specifications to facilitate and support clinical and laboratory research. Bioanalysis provides development, validation and implementation of high-performance liquid chromatography (HPLC)-based analytical methods for quantitative measurement of drugs, metabolites, or endogenous compounds in biological samples (including biofluid, tissue and cell culture samples). In addition, a broad range of pharmacology support is provided, including: 1) pharmacokinetic study design; 2) pharmacokinetic data analysis and modeling using traditional compartmental and non-compartmental analysis, nonlinear mixed-effect (population) pharmacokinetic modeling, and physiologically based pharmacokinetic modeling; 3) in vitro drug metabolism studies; 4) metabolite identification; and 5) determination of drug plasma protein binding and plasma-to-blood ratio. The Core is equipped with state-of-the-art analytical instruments (such as the AB SCIEX QTRAP 6500 LC- MS/MS system) and pharmacokinetic analysis software. The laboratory is centrally located with convenient access for all KCI investigators. The services provided by the Pharmacology Core have contributed to 92 peer-reviewed publications during the current review period.