Flow Cytometry and High Throughput Screening Shared Resource

流式细胞术与高通量筛选共享资源 摘要 UNMCC流式细胞术和高通量筛查共享资源（流式细胞术-HTS）提供 联合国赔偿委员会和其他研究人员拥有最先进的仪器、基础设施和专门知识。资源， 由布鲁斯爱德华兹博士指导，马克卡特管理，MS，提供细胞分析，细胞分选， 离线数据分析作为标准服务。资源用户接受仪器和软件方面的单独培训 使用.目前对资源用户的收费率在全国范围内处于中低水平。资源 也有创新的历史：UNMCC癌症治疗（CT）研究的资源和成员 程序（Sklar & B. Edwards）发明了使用流式细胞仪进行高通量筛选的能力， 平台（于2006年商业化为HyperCyt），这导致了一个成熟的高吞吐量的创建 筛选中心：UNM分子发现中心（CMD），隶属于资源。在 在过去的项目期间，该资源一直致力于为联合国赔偿委员会成员以及 其他NCI癌症中心的合作者和HyperCyt高通量筛查作为技术服务， 允许资源使用者常规访问新的高通量筛选技术。资源 支持小规模的筛查项目，针对那些已经开发出目标的研究人员， 在96或384孔板中筛选。该资源还充当地方政府资助的试点项目的渠道。 资源演变成更大的，NIH支持的筛选项目在CMD。这种高效的策略 使UNMCC项目成员能够识别雌激素受体的有效小分子探针， GTP酶，甲酰肽受体和外排转运蛋白，以及一些批准的药物，可能是 用于新的临床应用。该资源还提供了易于访问的预订和计费 系统.为了促进卓越的研究，该资源为UNCC方案成员和受训人员提供 专家的协助和信息，以促进细胞分析和发现项目的发展， 资助申请和出版物。一个资源优先事项是整合开发的先进技术， 将CMD和UNCC研究计划纳入其业务，使其更广泛地提供给 研究社区。教育和传播活动包括：参与制作《联合国墨西哥城共享 关于流式细胞术和药物相关专业主题的资源通讯、研讨会和演讲 探索，学生和教师的特设设施图尔斯之旅，以及一个资源网页，为即将举行的活动做广告 资源工具、服务和费用。在上一个5年项目期间，48名联合国赔偿委员会成员 来自4个联合国赔偿委员会研究方案的200多名研究人员使用了该资源，共出版了97份出版物，其中15份是 目前正在等待PMCID。在2013年7月至2014年6月的报告年度， 占总资源使用量的86%，并得到128项同行评审赠款的支持。

FLOW CYTOMETRY & HIGH THROUGHPUT SCREENING SHARED RESOURCE ABSTRACT The UNMCC Flow Cytometry and High Throughput Screening Shared Resource (Flow Cytometry-HTS) provides UNMCC and other researchers with state-of-the-art instruments, infrastructure and expertise. The Resource, directed by Bruce Edwards, PhD and managed by Mark Carter, MS, provides cell analysis, cell sorting, and offline data analysis as standard services. Resource users are individually trained in instrument and software use. Current charge back rates to Resource users are in the low to mid-range of rates nationwide. The Resource also has a history of innovation: The Resource and members of the UNMCC Cancer Therapeutics (CT) Research Program (Sklar & B. Edwards) invented the capability to conduct high throughput screens using a flow cytometric platform (commercialized as HyperCyt in 2006), which led to creation of a full-fledged High Throughput Screening Center: The UNM Center for Molecular Discovery (CMD), which is affiliated with the Resource. In the past project period, the Resource has worked to facilitate access to CMD for UNMCC members as well as collaborators at other NCI Cancer Centers and HyperCyt high throughput screening as a technical service, allowing Resource users routine access to novel high throughput screening technologies. The Resource supports small scale screening projects for investigators who have already developed targets that are ready for screening in 96- or 384-well plates. The Resource also serves as a conduit for pilot projects funded from local resources to evolve into larger, NIH-supported screening projects in the CMD. This highly productive strategy has allowed UNMCC Program members to identify potent small molecule probes for estrogen receptors, GTPases, formyl peptide receptors and efflux transporters, as well as a number of approved drugs that could be repurposed for new clinical applications. The Resource also provides easily accessible reservation and billing systems. To promote excellence in research, the Resource provides UNMCC Program members and trainees with expert assistance and information to facilitate development of cell analysis and discovery projects in support of grant applications and publications. A Resource priority is to integrate advanced technologies developed by the CMD and UNMCC Research Programs into its operations to make them more broadly available to the research community. Education and dissemination activities include: participation in production of a UNM Shared Resource newsletter, workshops and presentations on specialized topics pertaining to flow cytometry and drug discovery, ad hoc facility tours for students and faculty, and a Resource web page to advertise upcoming events and Resource instruments, services and fees. During the previous 5-yr project period, 48 UNMCC members from 4 UNMCC Research Programs used the Resource, resulting in a total of 97 publications, of which 15 are presently pending PMCIDs. In the reporting year of July 2013 – June 2014, UNMCC members were responsible for 86% of total Resource usage and were supported by 128 peer-reviewed grants.