LSM880-Airyscan Confocal Microscope for Large Shared Resource

 描述(由申请人提供)：我们申请资金购买基于Axio观察者Z1倒置机身的卡尔·蔡司LSM880和AiryScan共焦显微镜。主要目的是更换一辆使用了12年的蔡司LSM510Meta，这辆车是由Vanderbilt基金收购的，并于2003年整合到Vanderbilt细胞成像共享资源(CISR)中。配备AiryScan的LSM880在范德比尔特大学将是独一无二的，对于支持由NIH资助的、代表范德比尔特大学11个系的8个主要用户和10个次要用户组的正在进行的研究至关重要。这些研究人员在广泛的研究中需要先进的细胞解剖学和生理学成像，包括神经病理学和青光眼、神经元存活、肥胖和糖尿病、囊泡运输和癌症、心律失常、膜生物物理学、上皮形态发生和癌症以及肠道神经系统的神经脊谱系。我们预计，NIH资助的许多其他项目将得到该仪器的良好支持；特别是那些需要活细胞和组织成像的项目。基于三个主要目标，我们选择配备Airycan的LSM880作为最佳仪器：1)提高活细胞和组织成像的能力；2)提高复杂的多通道荧光显微镜和其他先进技术的高分辨率成像能力；3)保持与大量对共聚焦显微镜有基本需求的正在进行的研究的连续性。所要求的仪器是根据主要用户和次要用户的需求进行配置的，其中重点考虑了光学性能、在衍射极限下的高速图像采集，同时保持活细胞的生理条件。这些规格是成功实施主要用户项目中描述的先进共焦成像的基础。该仪器将设在医学研究大楼III(MRBIII室4155室)，由细胞成像共享资源(CISR)维护。这间屋子是一间为所有范德比尔特调查人员而设的显微镜设施，由该机构独家维护。这个空间是安全的，所有主要用户都可以轻松访问。对这一文书的专门知识和机构支持是特殊的。威尔斯博士是国家公认的显微镜专家，在共焦、多光子激发荧光和其他先进成像方法方面经验丰富。该研究所已有21年的历史，为300多个研究实验室提供尖端成像专业知识和合作研究支持。VUMC已承诺提供10万美元，支持该仪器的长期运行。

 DESCRIPTION (provided by applicant): We request funds to purchase a Carl Zeiss LSM880 with Airyscan confocal microscope based on an Axio Observer Z1 inverted body. The main aim is to replace a heavily used 12-year old Zeiss LSM510 Meta, acquired with Vanderbilt funds and integrated into the Vanderbilt Cell Imaging Shared Resource (CISR) in 2003. The LSM880 with Airyscan will be unique at Vanderbilt and crucial to support the ongoing NIH-funded research of 8 Major User and 10 Minor User groups representing 11 departments within Vanderbilt University. These investigators have essential needs for advanced imaging of cellular anatomy and physiology in a wide range of investigations including, neuropathology and glaucoma, neuronal survival, obesity and diabetes, vesicle trafficking and cancer, cardiac arrhythmia, membrane biophysics, epithelial morphogenesis and cancer, and neural crest lineages in the enteric nervous system. We expect that numerous other NIH-funded projects will be well supported by this instrument; particularly those that require live cell and tissue imaging. We chose the LSM880 with Airyscan as the optimal instrument based on three main goals: 1) to improve the capabilities for live cell and tissue imaging, 2) to improve the capabilities for high-resolution imaging of complex, multi-channel fluorescence microscopy and other advanced techniques, and 3) to maintain continuity with a large number of ongoing investigations with essential needs for confocal microscopy. The requested instrument is configured based on the needs of the Major and Minor Users with significant consideration given to optical performance, high-speed image acquisition at the diffraction-limit, while maintaining live cell physiological conditions. The specifications are fundamental to the successful implementation of the advanced confocal imaging described in the Major User projects. The instrument will be located in Medical Research Building III (room 4155 MRBIII), and maintained by the Cell Imaging Shared Resource (CISR). This room is a CISR microscopy facility, maintained exclusively by the CISR, for the benefit of all Vanderbilt investigators. This space is secure and easily accessible to all major users. The expertise and institutional support for this instrument are exceptional. Dr. Wells, Director of the CISR, is a nationally recognized microscopy expert with experience in confocal, multi-photon-excited fluorescence, and other advanced imaging methods. The CISR has a 21-year history of assisting over 300 investigator labs with cutting-edge imaging expertise and cooperative research support. The VUMC has committed $100,000 to support the long-term operation of the instrument.