High performance liquid chromatography for bio-active compounds research

需要一个具有半制备能力的高效液相色谱系统来支持Prithiviraj博士（海藻的Rispersphere植物-微生物相互作用/生物活性）和Rouvinen-Watt博士（n-3/n-6脂肪酸在水貂护理病、肥胖和脂肪肝疾病中的作用）的研究项目。该设备将补充两个NSERC发现赠款的研究活动，一个NSERC-AAFC-RPA和一个NSERC-CRD补助金申请。该设备还将用于实施申请人的项目，这些项目由新斯科舍省农业部和大西洋创新基金资助，此外，该设备的分析和半制备能力的可用性将有助于吸引来自联邦和省政府机构和行业的额外研究资金。所要求的配置符合两个研究项目的要求。该系统由配备四元泵的沃茨分离LC系统组成，能够处理多个移动的相的等度和梯度方案，这种能力对于有效分离复杂分析物（如根系分泌物、植物提取物、血清和动物组织样品）至关重要。根系分泌物、微生物分泌物、氨基酸和脂肪酸中存在的有机化合物在环境温度下容易降解，需要在低温下储存和分析。为了实现这一点，系统中包括沃茨717温控自动进样器。申请人需要两个检测器，即沃茨2996光电二极管阵列检测器（PDA）和沃茨2475多通道荧光检测器，因为这将使申请人能够将仪器用于多种功能。该HPLC系统将提高两个合作研究小组的分析能力和生产力。更重要的是，这些设备将在未来四年内用于培训23名高素质人员。

A high performance liquid chromatography system with semi-preparative capability is requested to support the research programs of Dr. Prithiviraj (Rhizosphere plant-microbe interaction / bioactivity of seaweeds) and Dr. Rouvinen-Watt (The role of n-3/n-6 fatty acids in mink nursing sickness, obesity and fatty liver disease). This equipment will complement research activities of two NSERC discovery grants, a NSERC-AAFC-RPA and a NSERC-CRD grant application. This equipment will also be used to carry out applicant's projects funded by Nova Scotia Department of Agriculture and Atlantic Innovation Fund. Further, availability of the analytical and semi-preparative capability of this equipment will help attract additional research funding from federal and provincial government agencies and industries. The configuration requested fits the requirements of both research programs. The system consists of a Waters Preparative LC System with a Quartenary pump capable of handling isocratic and gradient protocols with multiple mobile phases, this capability is essential to effectively fractionate complex analytes like root exudates, plant extracts, serum and animal tissue samples. Organic compounds present in root exudates, microbial secretions, amino acid and fatty acid are susceptible to degradation at ambient temperatures and required to be stored and analysed at low temperatures. To achieve this a Waters 717 temperature controlled autosampler is included in the system. Two detectors, a Waters 2996 photo diode array detector (PDA) and a Waters 2475 multichannel fluorescence detector, are requested as it will enable the applicants to use the instrument for multiple functions. This HPLC system will enhance the analytical capability and productivity of two collaborating research groups. More importantly, this equipment will be used to train 23 Highly Qualified Personnel (HQPs) during the next four years.