photo-initiated entrapping of chromatographic particles for high resolution microfluidic and capillary based seperation

蛋白质组学和基因组学研究为各种类型的分析提供了大量的样本。当前的分析技术无法跟上当前的需求速度，从而造成了分析瓶颈。微流体，极小体积的流体操作被视为一个可能的解决方案，以增加分析积压。在如此小的范围内处理液体对于分析化学领域具有许多优点，包括能够以大大提高的速度对小到几皮升的样品进行定性和定量分析。此外，由于微型化，与分析有关的大多数过程可以集成在单个微芯片内。本提案概述了一种新型光诱导制造工艺的发展，以固定商业试剂负载微球在毛细管和芯片上的实验室设备中。具有各种功能化表面的微球将用于化学分离、反应和增强检测。本提案中描述的新型微球/聚合物材料将使微球能够在设备内的任何地方进行照相图案化，大大简化了结构，并最终增加了在芯片内进行的分析类型。目标是将混合材料集成到低成本的聚合物设备中，以开发一种功能强大但价格低廉的蛋白质组学工具。

Proteomic and genomic studies have generated an enormous amount of samples for various types of analysis. Current analytical techniques are not able to keep up with the current rate of demand creating an analysis bottleneck. Microfluidics, the manipulation of extremely small volumes of fluids is seen as a possible solution to mounting analysis backlog. The handling of liquids on such a small scale has a number of advantages for the field of analytical chemistry including the ability to qualitatively and quantitatively analyze samples as small as a few picoliters at greatly enhanced speeds. Also as a result of miniaturization most processes involved with the analysis can be integrated within a single microchip. This proposal outlines research into the development of a novel light induced fabrication process to immobilize commercial reagent laden microspheres in capillaries and lab-on-a-chip devices. The microspheres, with various functionalized surfaces, will be used to perform chemical separations, reactions and enhance detection. The novel microsphere/polymer materials described within this proposal will enable microspheres to be photo-patterned anywhere within a device greatly simplifying construction and will ultimately increase the types of analyses conducted within a chip. The goal is to integrate the hybrid materials into low cost polymeric devices to develop a powerful, yet inexpensive proteomic tool.