Structural biology facility

要求拨款购买立体显微镜和制备离心机。准备离心机将用于收获含有重组蛋白的细胞培养物，并从细胞碎片中分离重组蛋白。在结晶过程中，立体显微镜将用于观察蛋白质晶体。收集细胞，分离重组蛋白，最重要的是观察蛋白质晶体的能力是NSERC发现研究项目成功的基础。该研究计划的重点是使用大分子晶体学来表征酵母中的去泛素化，包括测定泛素特异性蛋白酶的三级结构以及表征泛素特异性蛋白酶与其靶蛋白之间相互作用的机制。这种设备将提供给该部的其他成员，并将有助于训练高度合格的人员。

Funds to purchase a stereomicroscope and a preparative centrifuge are requested. The preparative centrifuge will be used to harvest cell cultures containing recombinant proteins and to separate recombinant proteins from cellular debris. The stereomicroscope will be used to observe protein crystals during crystallization setups. The abilities to harvest cells, separate recombinant proteins and most importantly to observe protein crystals is fundamental for the success of the NSERC Discovery research project. The focus of the research program is to use macromolecular crystallography to characterize deubiquitination in yeast including the determination of tertiary structures of ubiquitin specific proteases and the characterization of the mechanism of interaction between ubiquitin specific proteases and their target proteins. This equipment will be made available to other members of the department and will be instrumental in the training of highly qualified personnel.