DNA analysis infrastructure

我的研究计划的目标是：（一）记录自然种群内和自然种群之间的进化分歧模式，（二）测试关于从独特种群到物种的生物多样性起源的假设，以及（三）将这种理解应用于生物多样性的保护。在进化生物学中用来解决这些关键问题的模型是北美洲西北部的鱼类，包括：在不同的冰川避难所中隔离的进化谱系（棘鱼，鳟鱼和红点鲑）;通过物理地形显示出不同程度的物理分离的种群（例如，迁徙障碍、鳟鱼、红点鲑和灰鲭的流域相互联系）或生态学（鳟鱼和棘鱼对不同生境的适应）;以及在冰后期接触过的密切相关鱼类（红点鲑和棘鱼）。该研究计划的新颖之处包括：融合历史和当代的分歧过程，比较分析这些过程在旧的（例如，char）和更近的（例如，刺鱼）的分歧，以及在假设检验中分子和生态学方法的整合。 我们的工作对于在加拿大非常需要的领域-水生和分子生态学以及生物多样性研究-培训青年科学家非常重要。 这项研究计划的基础是分析从整个北美西北部收集的鱼类中获得的DNA。这项提案寻求资金来消除衰老，但这是提取和初步操纵DNA的关键设备。

The goals of my research program are to: (i) document the patterns of evolutionary divergence within and among natural populations, (ii) test hypotheses concerning the origins of biodiversity from unique populations to species, and (iii) apply such understanding to the conservation of biodiversity. The models used to address these key questions in evolutionary biology are fishes of northwestern North America, including: evolutionary lineages that were isolated in distinct glacial refugia (sticklebacks, trout, and char); populations that show various degrees of physical separation via physical landform (e.g., migration barriers, watershed interconnectedness in trout, char, and grayling) or ecology (adaptation to distinct habitats in trout and sticklebacks); and species of closely related fishes that have come into contact (char and sticklebacks) postglacially. The novel aspects of this research program include: integration of historical and contemporary processes in divergence, comparative analyses of these processes in old (e.g., char) and more recent (e.g., sticklebacks) divergences, and the integration of molecular andecological methods in hypothesis testing.  Our work is important for the training of young scientists in areas of great need for Canada - aquatic and molecular ecology and biodiversity research.  Fundamental to this research program is the analysis of DNA obtained from fishes collected from throughout northwestern North America. This proposal seeks funds to refurbish aging, but essential equipment central to the extraction and initial manipulation of DNA.