Digital imaging of developing organisms

了解生物体如何发育是理解生物体如何发挥功能的关键一步。了解动物发育的关键领域之一需要有能力人工调节基因或蛋白质的表达，然后测试其对胚胎或动物发育的影响。这可以通过一系列方法来完成，包括使用基因突变或扰乱蛋白质生产或改变发育信号发生方式的方法。然而，无论要研究的是哪种生物体，或扰乱发育过程的方式，可视化对胚胎发育的影响的能力是这种方法的关键部分。对于这些方法，需要对发育中的有机体或组织进行成像。研究人员及其要求这种设备的实验室研究发育的不同方面。然而，他们都使用类似的方法，利用抗体与组织染色相结合，评估蛋白质表达、信号传递的变化。细胞命运或形态。这种高分辨率显微镜是所有发育生物学实验室必不可少的一部分。许多被分析的发育缺陷都是单细胞或发育中组织的规模，因此使用了高倍率和优秀的光学和透镜。再加上使用这些高质量的显微镜，需要捕捉这些图像，以便进一步分析并将数据呈现给该领域的其他人。因此，这需要一台高端数码相机与计算机和软件一起运行这项拨款申请中所要求的相机。要求能够以高分辨率捕捉大量数据，以逼真地表示通过显微镜观察到的数据。此外，数码相机需要一台装有必要软件的计算机来运行相机，存储捕获的数据并分析生成的图像。

Understanding how an organism develops is a critical step in understanding how an organism functions.  One of the key apsects of understanding animal developmen requires the ability to manuipulate gene or protein expression and then test the effects on embryonic or animal development.  This can be done in a range of ways including the use of genetic mutations or methods that disrupt protein production or change how developmental signaling occurs.  However, no matter the organism to be investigated or the manner of disrupting developmental processes, the ability to visualize the effects on embryonic development is a vital part of this approach.  For these approaches imaging of developing organisms or tissues is required.  The researchers and their laboratories requesting this equipment study different aspects of development.  However they all use similar approaches that make use of antibodies to developmentally regulated  proteins paired with tissue staining to assess changes in protein expression, signaling, cell fate or morphology.  For this high resolution microscopes are an essential part of all developmental biology laboratories.  Many of the developmental defects analyzed are on the scale of single cells or developing tissues and thus high magnification paired with excellent optics and lenses are used.  Paired with the use of these high quality microscopes is the need to capture these images for futher analysis and presentation of the data to others in the field.   This requires a high end digital camera paired with a computer and software to rune the camera that has been requested in this grant applicaiton.  A digital camera has been requested that can capture large amounts of data with high resolution to faithfully represent the data observed through the microscope.  In addition the digital camera requires a computer with the necessary software to run the camera, store the captured data and analyze the generated images.