Gas chromatography-mass spectrometer for hormone quantitation

我的NSERC资助的研究包括了解植物激素如何调节植物生长和发育协调所涉及的生理和生化过程。为了研究激素的相互作用，并对植物激素（特别是生长素、赤霉素和脱落酸）如何相互作用以协调植物各组织之间的生长过程和响应不断变化的环境条件提出有效假设，对内源激素、激素前体和分解代谢产物进行明确的鉴定和定量是绝对必要的。质谱是唯一的技术，提供绝对的化合物鉴定和可重复的定量分析需要进行有效的研究。气相色谱与质谱联用（GC-MS）是定量分析植物激素及其前体和分解代谢物的理想方法，这些激素是我的NSERC研究项目和共同申请人的项目感兴趣的。韦斯莱克和斯特雷科夫。

My NSERC funded research involves understanding how plant hormones regulate physiological and biochemical processes involved in the coordination of plant growth and development. In order to study hormonal interactions and develop working hypotheses on how plant hormones (specifically auxins, gibberellins, and abscisic acid) interact to coordinate plant growth processes between various plant tissues, and to respond to changing environmental conditions, definitive identification and quantitation of endogenous hormones, as well as hormone precursors and catabolic products are absolutely necessary. Mass spectrometry is the only technique that provides absolute compound identification and reproducible quantitative analysis needed to undertake this research effectively. The combination of gas chromatography coupled with a mass spectrometer (GC-MS) is ideal for quantitative analysis of plant hormones, and their precursors and catabolites studied in my NSERC research program and those of interest in the programs of the co-applicants, Drs. Weselake and Strelkov.