The effect of cyclic strain and pressure on endothelial cell response

心脏病发作、中风、动脉瘤和瓣膜紊乱等心血管疾病被认为在一定程度上是细胞对生物机械力的反应。我的实验室已经开发出使用三维组织培养模型的技术，以更好地研究内皮细胞、白细胞和血小板对真实血流动力学的反应。目前，由于我们对刚性墙体力学的假设，这些模型仅限于将剪应力作为刺激因素进行研究。在体内，动脉壁是顺应性的，特别是在冠状动脉的情况下，在心脏周期中被周围组织拉和拉伸。这项拟议工作的目标是在我们现有的动态细胞培养模型中增加循环应变和顺应性。具体地说，我们将回答以下问题：1.血管顺应性和循环应变是否会改变内皮细胞的局部反应？2.顺应性和循环应变是否会影响血液成分与内皮的捆绑和附着？这项研究将提供对动脉粥样硬化的更好的了解，并最终导致更好的治疗方案。

Cardiovascular diseases such as heart attacks, strokes, aneurysms and valvular disorders are believed to be partly a cellular response to biomechanical forces. My laboratory has developed the techniques to use three dimensional tissue culture models to better investigate the response of endothelial cells, leukocytes and platelets to realistic hemodynamics. Currently these models are limited to the study of shear stress as a stimulus owing to our assumption of rigid wall mechanics. In vivo, the artery wall is compliant and, particularly in the case of coronary arteries, pulled and stretched by surrounding tissue during the cardiac cycle. The objective of the proposed work is to add cyclic strain and compliance to our existing dynamic cell culture models. Specifically, we will answer the questions: 1. Does vessel compliance and cyclic strain alter the local response of endothelial cells? 2. Is the tethering and attachment of blood components to the endothelium affected by compliance and cyclic strain? This research will provide a better understanding of atherosclerosis and ultimately lead to better treatment options.