Multi-modality opticalimaging in tissues using ultrafast lasers

癌症在北美所有死亡原因中排名第二。如果在浸润前阶段检测到，其存活率可明显提高。光学成像提供了一种非侵入性的方式来可视化和检测癌前病变和癌性病变。多光子显微术（MPM）和光学相干断层成像（OCT）是两种前沿的成像技术。MPM以亚微米分辨率和分子特异性检测细胞和细胞外基质。OCT提供以毫米尺度示出组织的解剖结构的横截面断层摄影图像。我们建议开发一种多模态光学成像系统，将MPM和OCT集成在一个平台上。MPM对细胞和细胞外基质的敏感性，以及OCT对界面的敏感性，可以在新血管形成、细胞迁移和细胞外基质重塑过程中对细胞-细胞和细胞-基质相互作用进行新的观察。这些事件对几乎所有生物过程都至关重要，从癌症到伤口愈合，衰老和糖尿病。MPM/OCT将提供一种新的成像工具，具有检测癌前病变和癌性病变的灵敏度和特异性。

Cancer ranks second among all causes of death in North America. If detected in the pre-invasive stage, its survival rate can be significantly increased. Optical imaging provides a noninvasive way to visualize and detect precancerous and cancerous lesions. Multiphoton Microscopy (MPM) and Optical Coherence Tomography (OCT) are two cutting-edge imaging techniques. MPM detects cells and extracellular matrix with sub-micron resolution and molecular specificity. OCT provides cross-sectional tomographic images showing anatomic structures of tissues on a millimeter scale. We propose to develop a multi-modality optical imaging system, integrating MPM and OCT on a single platform. The sensitivity of MPM to cells and extracellular matrix, and that of OCT to interfaces can enable new observations of cell-cell and cell-matrix interactions during the development of neovasculature, cell migration, and extracellular matrix remodeling. These events are fundamentally important to nearly all biological processes, from cancer to wound healing, aging, and diabetes. With benefits from both sides, MPM/OCT will provide a new imaging tool that has both sensitivity and specificity to detect precancerous and cancerous lesions.