Polymer synthesis of biomaterials for restoring hard and soft tissue function in oral-facila structures

这项发现基金的基本假设是，口腔液的模型酯酶生物降解系统可以定义聚合物生物材料结构，从而能够设计出生物稳定的牙齿修复材料，以及具有肽功能的生物可降解支架，用于再生功能性粘膜组织。这项发现基金将有三个主题。PI之前的工作已经确定了口腔唾液中最有效的酶复合体，这些复合酶导致了用于牙齿修复的传统聚合物复合材料的生化分解。更新发现基金的目标是使用上述酶复合体的模型酶系统有条不紊地鉴定可用于构思高生物稳定性和抗菌牙齿修复材料的新单体结构。在另一项工作中，研究人员的实验室已经证明，通过从复合聚合物释放低ppm到ppb水平的降解产物，在分子水平上调节细菌的生物功能是可能的。因此，这笔续展发现赠款将探索合成新的单体，以生成能够释放抗菌剂的复合材料，同时发挥其作为牙齿填充的功能。最后一项研究主题将探索可支持粘膜软组织细胞的微孔支架结构的制备。这项工作将重点介绍3个基本设计参数，这些参数被认为是口腔软组织再生的最终目标所必需的，包括单体转化率、生物降解率和三维支架内的细胞增殖。这些材料最终可能被用来修复因年龄、遗传和环境相关疾病而受损的组织。这笔赠款的发现将提供更多关于单体化学结构对生物材料的物理、生化和生物结果的影响的机械理解，旨在用于对加拿大人具有物理、心理/社会和经济影响的与健康相关的领域(即口腔健康科学)。这些聚合物还可能在农业和环境管理系统的生物递送概念中具有潜在的用途。

The underlying hypothesis of this discovery grant is that model esterase biodegradation systems for oral fluids can define polymeric biomaterial structures that will enable the design of biostable tooth restorative materials, as well as biodegradable scaffolds with peptide function for regenerating functional mucosal tissues. There will be 3 main themes to this discovery grant. Previous work by the PI has identified the most potent enzyme complexes in oral salivary fluids which are responsible for the biochemical breakdown of traditional polymeric composite materials used in tooth restorations. It is an objective of the renewal discovery grant to use model enzyme systems for the above enzyme complexes to methodically identify novel monomeric structures that could be used to conceive highly biostable and antimicrobial tooth restorative materials. In other work the investigator's lab has demonstrated that it is possible to modulate the biological function of bacteria at the molecular level with the release of low ppm to ppb levels of degradation products from composite polymers. Therefore, this renewal discovery grant will explore the synthesis of new monomers to generate composite materials which could release antimicrobial agents while fulfilling their functional role as a tooth filling. The last research theme will explore the fabrication of micro-porous scaffold structures that could support mucosal soft tissue cells.This work will emphasis 3 principle design parameters, believed to be essential in the ultimate goal of regenerating soft tissue in the oral cavity, including monomer conversion, biodegradation rates and cell proliferation within the 3-dimensional scaffolds. These materials may ultimately be used to restore tissue damaged by age, genetic and environmental related illnesses. The findings from this grant will provide a greater mechanistic understanding for the influence of monomer chemical structure on the physical, biochemical and biological outcomes of biomaterials, intended for a health related area (i.e. the oral-health sciences) that has physical, psychological/societal, and economic impact on Canadians. These polymers may also have potential uses in biological delivery concepts for agriculture and environmental management systems.