Discovery Projects - Grant ID: DP210101957

单次平行线突变导入（SIMPL）。该项目旨在开发一种新的方法，以显着加快生产转基因小鼠，这是一个关键的“工具”，研究生物过程和疾病。这项工作计划将最新的基因编辑技术CRISPR提升到一个新的水平，开发一种基于CRISPR的新方法，以目前成本和时间的一小部分产生具有相同基因序列不同变异的不同小鼠品系。该项目将克服以前所未有的细节研究基因功能的主要障碍，从而为未来研究生物过程开辟新的途径。因此，该项目的成果将影响整个生物医学研究领域，并推动澳大利亚的生物技术产业。

Single-session Introduction of Mutations in Parallel Lines (SIMPL). This project aims to develop a novel method for markedly accelerating production of genetically modified mice, which are a key 'tool' for studying biological processes and diseases. The work plans to take CRISPR, the latest gene-editing technique, to the next level by developing a novel CRISPR-based method to generate different mouse strains with distinct variations of the same gene sequences, at a fraction of the present cost and time. This project should overcome a major barrier to studying gene function with unprecedented detail, thereby opening new avenues for future research into biological processes. Thus, the outcomes from this project should impact on the entire field of biomedical research, and advance Australia's biotech industry.