Identifying Novel Biosynthetic Pathways in Mycobacteria using DNA Microarray Technology

利用DNA芯片技术鉴定分枝杆菌新的生物合成途径。DNA微阵列是一种强大的基于生物信息学的新技术，是表征复杂生物合成途径的理想工具，因为细菌基因组中所有基因的表达可以在单个实验中进行监测。在这个项目中，我们的目标是构建和使用DNA微阵列来识别分枝杆菌中新的生物合成途径。特别感兴趣的是用于创建高度复杂和特征不佳的细胞壁成分的途径。由于这种结构在细菌世界中是独特的，我们期望识别和表征分枝杆菌特有的途径。

Identifying Novel Biosynthetic Pathways in Mycobacteria using DNA Microarray Technology. DNA microarrays are a powerful new bioinformatics-based technology and an ideal tool for characterising complex biosynthetic pathways since the expression of all genes in the bacterial genome can be monitored in a single experiment. In this project we aim to construct and use a DNA microarray to identify novel biosynthetic pathways in mycobacteria. Of particular interest are pathways used to create components of the highly complex and poorly characterised cell wall. Since this structure is unique in the bacterial world, we expect to identify and characterise pathways that are unique to mycobacteria.