Imaging system for quantification of DNA, RNA, and proteins

一个由多种相互作用途径组成的复杂网络允许活细胞利用周围环境中的营养物质来适应环境变化。这种出色的适应能力需要协调信号感知、信号转导、基因表达调节以及最终调节细胞代谢。因此，定量分析对于理解和剖析正常生理状态和疾病的基本机制至关重要。因此，我们的研究迫切需要所需的成像系统来定量分析核酸（DNA和RNA）和蛋白质。对于关键调控步骤的分析，我们使用放射学和荧光方法来精确测量变化的量和时间，以及DNA，RNA和蛋白质的修饰。特别是，所要求的设备将用于研究各种分子过程，包括细菌感染机制，转录调节，染色质重塑，植物-病原体相互作用和心血管疾病。所要求的成像系统的收购是必不可少的，我们的研究，因此，预计将提供根本性的进步，在细胞信号传导和基因表达的几个调控步骤的理解。

A complex network of diverse interplaying pathways allows living cells to use nutrients from their surroundings to adapt to environmental changes. This outstanding capacity for adaptation requires the orchestration of signal perception, signal transduction, gene expression regulation and ultimately the adjustment of cellular metabolism. Therefore, quantitative analysis is of critical importance to understand and dissect basic mechanisms in normal physiological states and diseases. Accordingly, our research critically requires the requested imaging system for quantitative analyzes of nucleic acids (DNA and RNA) and proteins. For the analysis of key regulatory steps, we use radiographic and chemiluminescent approaches to precisely measure the amount and timing of changes, as well as the modification of DNA, RNA, and proteins. In particular, the requested equipment will be used to study various molecular processes including bacterial infection mechanisms, transcription regulation, chromatin remodeling, plant-pathogen interactions and cardiovascular diseases. The acquisition of the requested imaging system is essential to our research, and is therefore expected to provide fundamental advancement in the understanding of several regulatory steps of cell signaling and gene expression.