To assess biological properties of yellow mustard mucilage on skin cells in vitro

黄芥胶对皮肤细胞生物学特性的体外评价 加拿大是全球最大的黄芥末(Sinapis Alba L.)出口国，黄芥末主要用于烹饪和工业食品生产。黄芥末在不同的芥末品种中是独一无二的，因为它的麸皮含有丰富的粘液；因此，黄芥末粘液(MM)在食品工业以外的其他用途将为芥末行业带来额外的经济利益。 我们的团队由NSERC支持的三名申请者组成，他们拥有有效提取MM方法的专业知识和专有权，他们进行了一项初步研究，发现MM对突变的但不是正常的人类皮肤细胞具有一种新的生长抑制活性。在目前的项目中，我们希望验证我们最初的观察，并使用更大的正常和突变的人类皮肤成纤维细胞、黑素细胞和角质形成细胞来建立原理证明。由于这些异常皮肤细胞携带P53、B-raf、PTEN和N-ras等基因突变，这些基因控制着多种细胞类型的生长和死亡，我们将评估MM对细胞存活、细胞周期和细胞死亡相关参数的影响，以表征MM对突变皮肤细胞的优先作用。我们还将研究MM诱导的这些细胞代谢组学的变化，以确定与MM的生长抑制功能有关的代谢途径。我们的基本观察结果将为MM在食品工业以外的未来更高附加值的应用提供一个平台。 我们的行业合作伙伴Mustard 21 Canada Inc.为我们的计划提供了坚定的支持，因为它有可能为加拿大各地的芥末种植者带来好处。因此，我们的研究计划提供了在产学合作环境中广泛培训高素质人员的机会，旨在确定基础研究活动的有益行业应用。

To assess biological properties of yellow mustard mucilage on skin cells in vitro Canada is the number one global exporter of yellow mustard (Sinapis alba L.), which is used mainly in cooking and industrial food production. Yellow mustard is unique among the different mustard varieties by its bran being rich in mucilage; hence other uses of yellow mustard mucilage (MM) outside the food industry would bring additional economic benefits to mustard industry. Our team of three NSERC-supported applicants, which has the expertise and proprietary rights for an efficient method of extraction of MM, conducted a preliminary study and identified a novel growth-suppressive activity of MM on mutant but not normal human skin cells. In the present project, we wish to validate our initial observation and establish a proof of principle using a larger panel of normal and mutant human skin fibroblasts, melanocytes and keratinocytes. Since these abnormal skin cells carry mutations in genes such as p53, B-raf, PTEN and N-ras, which control growth and death in multiple cell types, we will assess the effect of MM on cell survival, cell cycle and cell death-related parameters to characterize the preferential effect of MM on mutant skin cells. We will also examine MM-induced alterations in metabolomics profile of these cells to identify metabolic pathways implicated in growth-suppressive function of MM. Our fundamental observations will provide a platform for future higher value-added applications of MM beyond the food industry. Our industry partner Mustard 21 Canada Inc. has provided a firm support to our program because of its potential to bring benefits to mustard growers across Canada. Thus, our research program offers an opportunity for extensive training of highly qualified personnel in an industry-university collaborative environment aimed at identifying the beneficial industrial applications of fundamental research activities.