A biosurfactant aided composting approach and associated microarray analysis

. Nordlys Environmental，LP（“Nordlys”）已选择堆肥作为易地处理的主要方法。 处理位于5翼的救生舱内受石油烃污染的泥土 拉布拉多的鹅湾。然而，堆肥处理PHC污染土壤的应用面临着几个问题， 挑战首先，由于不同的场地条件，处理效率有很大的差异。 为进行STF补救，需要根据具体的土壤条件进行设置。其次，由于低 为了提高PHCs的生物利用率，需要确定适当的堆肥改良剂， 加速治疗过程。第三，了解微生物群落和代谢 在堆肥过程中的路径仍然非常有限，并阻碍了有效的过程控制。 拟议的项目旨在通过开发生物表面活性剂辅助的 堆肥方法和进行相关的微阵列分析的土壤样品从STF。项目 将：（1）开发实验室规模的堆肥系统，以获得优化的操作条件，以有效地， 和成本效益，处理特定地点的污染土壤;（2）应用一种新型的生物表面活性剂作为修正剂， 提高PHCs的生物有效性，加快堆肥化进程;（3）微生物研究 堆肥修复中的群落动态、代谢途径及相应的功能基因 系统使用GeoChip 5.0，基于微阵列的宏基因组学工具，并将结果与堆肥过程相关联 控制参数这项研究将为PHC的处理提供一种新的堆肥方法 污染的土壤中。它还将大大扩展堆肥相关微生物的科学知识 社区.通过与Nordlys的合作，拟议的项目将提供一个实用和创新的 解决公司现有问题的解决方案，为公司带来长期利益， 加拿大，通过技术转让和实地应用。

. Composting has been selected by Nordlys Environmental, LP ("Nordlys") as a main approach in the ex-situ treatment of petroleum hydrocarbon (PHC) contaminated soil in the Survival Tank Farm (STF) at 5 Wing Goose Bay in Labrador. However, the application of composting to treat PHC contaminated soil faces several challenges. Firstly, wide variations of treatment efficiencies have been reported due to diverse site conditions. Settings tailored to specific soil conditions need to be obtained for STF remediation. Secondly, due to low bioavailability of PHCs, appropriate composting amendments need to be identified for increasing the rate of, and accelerating, the treatment process. Thirdly, the understanding of microbial communities and metabolic pathways during composting is still extremely limited and has hindered effective process control. The proposed project aims to address this knowledge and technical gap by developing a biosurfactant-aided composting approach and conducting associated microarray analyses on soil samples from STF. The project will: (1) develop a laboratory-scale composting system to obtain optimized operation conditions to efficiently, and cost-effectively, treat site-specific contaminated soil; (2) apply a novel biosurfactant as the amendment to increase the bioavailability of PHCs and accelerate the composting treatment; and (3) investigate microbial community dynamics, metabolic pathways and corresponding functional genes in the composting remediation system using GeoChip 5.0, a microarray-based metagenomics tool, and relate the results to composting process control parameters. The proposed research will lead to a novel composting approach for the treatment of PHC contaminated soil in STF. It will also greatly expand scientific knowledge on composting-associated microbial communities. With the collaboration with Nordlys, the proposed project will provide a practical and innovative solution that addresses the company's existing problems, generating a long-term benefit to the company, and Canada, through technology transfer and field applications.