Proteomic mass spectrometry analysis of genetically engineered crops

商业蛋白质主要使用重组技术和成熟的生产平台生产，如微生物、酵母或哺乳动物细胞培养。随着植物转化技术的出现，植物和藻类已被证明是大规模生产重组蛋白的可行生物反应器。其优势在于生产成本、可扩展性和产品安全性、易于存储和分发，这些都是目前任何细菌或哺乳动物生产平台都无法比拟的。Proteins Easy Corp是一家加拿大生物技术开发公司，利用基因插入和选择性表达的专利工艺改造重组蛋白质的生产，然后进行专有温室繁殖和中试植物分离和纯化。在拟议的项目中，我们的目标是利用蛋白质组质谱来了解商业融合蛋白是如何与水稻细胞内生长的颗粒联系在一起的。我们将产生新的蛋白质组数据，作为公司基因工程师的重要资源，因为发现的大米蛋白质可以用于优化蛋白质DNA结构的优化设计

Commercial proteins are mainly produced using recombinant technology and established production platforms such as microbial, yeast, or mammalian cell cultures. With the advent of plant transformation technology, plants and algae have proven to be feasible bioreactors for the large-scale production of recombinant proteins. The advantages are in terms of production costs, scalability and product safety, ease of storage and distribution, none of which can be matched by any current bacterial or mammalian production platform. Proteins Easy Corp is a Canadian biotechnology development company transforming the production of recombinant proteins with patented processes for gene insertion and selective expression, followed by proprietary greenhouse propagation and pilot plant isolation and purification. In the proposed project we aim at understanding how a commercial fusion-protein becomes tethered to the granule growing inside rice cells using proteomic mass spectrometry. We will generate new proteomic data as an important resource for company's genetic engineers because the discovered rice proteins can be deployed in optimizing the design of improved DNA constructs for proteins