Smart polymer for antibody purification

涉及人类单抗的治疗策略正变得越来越普遍。因此，降低这些药物成本的愿望正在施加相当大的压力，以使生产过程更有效率和成本效益。然而，尽管生产抗体的细胞培养技术已经得到改进，产量超过5g/L，但从培养液中纯化抗体已经成为一个主要的工艺瓶颈。目前，使用色谱柱从细胞培养上清液中选择性地捕获抗体是最有效的抗体纯化方法。不幸的是，现有的柱极其昂贵，需要苛刻的清洗/再生过程才能重复使用，并且由于其固定在固体载体上，经常以不到化学计量比的量结合靶。为了解决这些缺点，BioAstra Technologies Inc.和INRS将开发一种可回收的清道夫，可以从细胞培养液中捕获和回收单抗，从而绕过了对层析的需要。该项目是在这一领域培训HQP的绝佳机会，具有重要的社会和经济意义。该项目的成果将是一项重大的技术进步，将降低单抗及其相应疗法的成本。

Therapeutic strategies involving human monoclonal antibodies are becoming increasingly prevalent. Thus, the desire to reduce the cost of these drugs is exerting considerable pressure to make the manufacturing process more efficient and cost effective. However, while cell-culturing technology to produce antibodies has improved to deliver yields greater than 5 g/L, purification of the antibody from the medium has become a major process bottleneck. Currently, the use of chromatography columns that selectively capture antibodies from cell culture medium is the most effective method for purifying antibodies. Unfortunately, existing columns are extremely expensive, require harsh cleaning/regeneration processes in order to be reused, and often bind the target in a less-than-stoichiometric amount due to its immobilization on a solid support. To address these shortcomings, BioAstra Technologies Inc. and INRS will develop a recyclable scavenger that can capture and retrieve monoclonal antibodies from cell culture medium, thereby bypassing the need for chromatography. This project represents an excellent opportunity to train HQP in this area, which is of great social and economic importance. The output of this project will constitute a major technological advance that will reduce the cost of monoclonal antibodies and their corresponding therapies.