The Procurement of BioAnalyzers to Expedite and Enhance Services Provided by the National Natural Toxins Research Center

项目总结： 天然产品一直是药物设计的基础，而蛇和其他动物的毒液具有巨大的 潜力。蛇毒提供了分子探针，已被用来破译许多 复杂的生理和病理生理过程，并已成为 开发几个重要的药物类别。蛇毒和抗蛇毒研究领域依赖于 获得国家天然毒素开发的可靠和可重复使用的毒液相关资源 研究中心(NNTRC)。NNTRC需要用于储存生蛇的设备和仪器 毒液、毒液组分、纯化毒素、克隆产品、蛇和老鼠组织、关键试剂(即， 生化分析和试剂、抗体、结合抗体和生物样品)用于 多样化的翻译研究。所要求的设备和仪器，两个超低 温度冰柜，Bio-Plex 200多路阵列系统，DxH 500血细胞分析仪，Stago 建议中的STA紧凑型凝血分析仪和DM1000 LED立式显微镜是理想的 适用于支持关键研究，以保持生物样本的长期储存，分析 多路形式的生化蛋白质标记，以更少的样本量提供更多信息 与传统的免疫分析方法相比，在更短的时间内测定血液学指标和 蛇类血液学特征异常的凝血指标 毒液。家长赠款的具体目标是： 1.将国家天然毒素研究中心作为资源中心运营，提供高质量的 优质毒液和产品，支持国家和地区的生物和生物医学研究 国际项目。 2.发展和扩大蛇的收藏、专门服务和外展计划，以 支持美国毒液相关研究的发展。 3.开展最先进的应用研究计划，以支持新毒液的开发- 相关研究服务。 获得这些设备和仪器将使NNTRC能够实现其具体目标，并允许 研究人员将在寻找毒液衍生疗法方面取得重大进展。 相关性： 毒液研究是一个多元化和多学科的领域，涵盖了一系列科学领域， 包括生物化学、药理学、生态学和保护生物学。它可能会导致这一发现 新药，更好的抗蛇毒药物，以及对有毒动物及其在 生态系统。NNTRC的资源已被广泛应用于这些领域的研究 学科，已得到多个NIH I/C、NSF、国防部和 国家和国际研究机构。

PROJECT SUMMARY: Natural products have been a foundation of drug design, and snakes and other animal venoms have vast potential. Snake venoms have provided molecular probes that have been used to decipher numerous complex physiological and pathophysiological processes and have served as the starting point for developing several important classes of drugs. The venom and anti-venom research fields depend on access to reliable and reproducible venom-related resources developed by the National Natural Toxin Research Center (NNTRC). The NNTRC requires equipment and instrumentation for storing crude snake venoms, venom fractions, purified toxins, cloned products, snake and mice tissues, critical reagents (i.e., biochemical assays and reagents, antibodies, conjugated antibodies, and biological samples) used for diverse translational research. The equipment and instrumentation being requested, two ultra-low temperature freezers, a Bio-Plex 200 Multiplex Array System, a DxH 500 Hematology Analyzer, a Stago STA Compact Coagulation Analyzer, and a DM1000 LED upright microscope, in the proposal are ideally suited to support critical research for maintaining the biological samples for long-term storage, analyzing biochemical protein markers in a multiplex format that provide more information from less sample volume in less time than traditional immunoassay methods, determining the hematological variables and coagulation parameters for the characteristic hematological abnormalities observed in snake envenomation. The specific aims of the parent grant are to: 1. Operate the National Natural Toxins Research Center as a resource center that provides high- quality venom and products that support biological and biomedical research to national and international programs. 2. Develop and expand the collection of snakes, specialized services, and outreach programs to support the growth of venom-related research in the U.S. 3. Conduct a state-of-the-art applied research program to support the development of new venom- related research services. Access to this equipment and instruments will enable the NNTRC to fulfill its specific aims and allow researchers to make significant advances in the search for venom-derived therapeutics. RELEVANCE: Venom research is a diverse and multidisciplinary field that encompasses a range of scientific areas, including biochemistry, pharmacology, ecology, and conservation biology. It could lead to the discovery of new drugs, better antivenoms, and an improved understanding of venomous animals and their role in ecosystems. The resources of the NNTRC have been applied to research in a wide range of these disciplines, research that has been supported by multiple NIH I/C’s, the NSF, the Dept of Defense, and national and international research agencies.