Feasibility for 'Real Time' Quantification of Wastewater Bacteriological Composition

从水质和人类健康的角度来看，“实时”病原体检测和量化的概念具有巨大的价值。这不仅从饮用水的角度来看是必要的，而且从废水排放的角度来看也是必要的。例如，这种方法可以通知公众泳滩/康乐用水通道、雨水径流排放、下水道溢水质量或抛光池塘的性能等。TECTA-PDS开发了世界上第一个自动化的，epa批准的微生物水质检测系统，使其更容易，更快，更便宜地进行现场水检测大肠杆菌。迄今为止，该技术主要集中于通过大肠杆菌、总大肠菌群和肠球菌的存在-不存在测试来确定饮用水的可携带性。现有技术仅限于废水应用的二进制结果，但是TECTA-PDS设备可以提供量化值。该项目旨在扩大对一系列特定于废水质量的细菌的可量化性。这将通过(1)确定TECTA是否是一种量化不同来源废水中细菌组成的可行方法来实现；(2)评估饮用水中的现有校准（大肠杆菌、总大肠菌群和肠球菌）是否适用于废水；(3)通过自定义校准建立适当的废水测试方案和TECTA参数。短期内，该项目将在6个月的时间内培养1名masc和2名本科HQP。积极的结果将促成后续的长期合作，包括实验规模，然后是与皇后大学贝蒂水研究中心的TECTA-PDS合作进行中试规模的测试。对小型、农村和偏远社区的细菌废水参数进行快速监测的能力可以帮助这些社区以低成本实现其管制废水排放要求。这反过来又有望改善对加拿大流域的保护，这些流域目前正受到超过监管要求的废水排放的影响。

The concept of a 'real time' pathogen detection and quantification holds tremendous value from a water qualityand human health perspective. This is essential not only from a drinking water perspective, but also from awastewater effluent discharge. For example, such an approach could inform public beach/recreational wateraccess, stormwater runoff discharge, sewer overflow quality or polishing pond performance to name a few.TECTA-PDS developed the world's first automated, EPA-approved microbiological water quality testingsystem, making it easier, faster and less expensive to do on-site water testing for E. coli bacteria. To date, thistechnology has primarily been focused on determining drinking water portability via presence-absence tests forEscherichia coli (E. coli), total coliforms and Enterococcus. The existing technology is limited to binary resultsfor wastewater applications, however TECTA-PDS equipment can provide quantifying values. This projectaims to broaden bacterial quantifiability on a range of bacteria specific to wastewater quality. This will beaccomplised by (1) determining whether TECTA is a viable method of quantifying bacteria composition inwastewater from different sources; (2) assessing whether existing calibrations (for E. coli, total coliforms andEnterococcus) in drinking water are suitable for wastewater; and (3) establishing appropriate wastewater testingprotocols and TECTA parameters via custom calibrations. In the short term, tthe project will train one MAScand two undergraduate HQP over a 6-month period. Positive results will enable subsequent collaborations, on alonger term basis, involving bench-scale, then pilot-scale testing in collaboration with TECTA-PDS at theBeaty Water Research Centre at Queen's University. The capacity for rapid monitoring of bacterial wastewaterparameters for small, rural and remote communities could assist these communities to achieve their regulatoryeffluent discharge requirements at a low cost. This in turn is expected to improve protection for Canadianwatersheds which are currently being impacted by wastewater discharge in exceedance of regulatoryrequirements.