Investigation and development of an accurate ethanol content measurement method for kombucha

Booch Inc.生产有机工艺康普茶，并将其产品分销给安大略省200多家零售商。Booch Inc.是安大略省最大的康普茶啤酒厂，也是加拿大第二大康普茶啤酒厂，在这个不断发展的行业中被公认为领导者和潮流引领者。康普茶是一种天然碳酸饮料，由红茶或绿茶、糖和各种细菌和酵母发酵而成。目前，市场上还没有准确的方法来测量康普茶酿造过程中的酒精含量。一些酿酒商甚至因为不准确地报告其产品中的酒精含量而遭受法律后果。Booch已经确定了一种潜在的新产品——康普茶乙醇含量测量试剂盒，该试剂盒将包括一份详细的协议，概述了在康普茶酿造过程中测量乙醇含量的经过验证的方法。为了探索这一潜在的新产品，Booch将与范肖学院应用科学与技术学院合作。一名学生研究助理将与首席研究员卡伦·布赫霍尔兹合作，开发并比较三种测量酿造康普茶中乙醇含量的方法。随着测量方法的发展，学生将调查哪种方法最有效，并准备一份详细说明所选方法的协议。这一应用研究项目的结果将帮助Booch将其客户市场扩展到其他康普茶酿造商。它将帮助Booch和其他康普茶酿造商做出决定，例如是否可以改变发酵条件以生产可接受的产品，或者是否应该放弃一批以节省时间和资源。此外，它将确保所有年龄段的康普茶产品都是安全的，而且这种饮料被归类为无酒精（低于0.05%）。表格103 (2011 W)，第7页

Booch Inc. manufactures organic craft kombucha and distributes their product to over 200 retailers acrossOntario. Operating for just over 2 years, Booch Inc. is the largest kombucha brewery in Ontario, second largestin Canada, and recognized as a leader and trendsetter within this growing industry. Kombucha is a naturallycarbonated beverage made by fermenting black or green tea and sugar with a culture of various bacteria andyeasts. Currently, there is no accurate method available on the market to measure alcohol content during thekombucha brewing process. Some brewers have even experienced legal repercussions due to inaccuratereporting of alcohol content in their product. Booch has identified a potential new product, a kombucha ethanolcontent measurement kit, which would consist of a detailed protocol outlining a proven method for measuringethanol content while kombucha is brewing. To explore this potential new product Booch will be collaboratingwith Fanshawe College's Applied Science and Technology School. Working with Principal Investigator, KarenBuchholz, a student research assistant will develop and compare three methods of measuring the ethanolcontent in brewing kombucha. Following development of the measurement methods, the student willinvestigate which method is most effective, and prepare a protocol detailing the selected method. The results ofthis applied research project will assist Booch in expanding their client market to other kombucha brewers. Itwill help Booch and other kombucha brewers in making decisions such as whether or not fermentationconditions can be altered to produce an acceptable product, or if a batch should be abandoned to save time andresources. Additionally, it will ensure safe kombucha products for all ages, and that the beverage is classifiedas non-alcoholic (less than 0.05%).3Form 103 (2011 W), page 3 of 7Second