Purchase of a preparative HPLC system for bioactive peptides research

食品蛋白质衍生的多肽仍然是发现有关生物活性物质结构和功能之间关系的基本科学知识的重要信息来源。这是因为在肽链长度(2-20个残基)的背景下，20种自然产生的氨基酸的可能组合几乎不是穷尽的。然而，由于多肽大小和链长的相似性，生物活性多肽的分离、纯化、功能表征和体内测试需要使用高压液相色谱(HPLC)系统进行定量分析。在马尼托巴大学食品与人类营养科学系，用于我的NSERC多肽发现研究的已有10年历史的制备型高效液相(Prep-HPLC)系统将在2018年7月后不再使用。这是因为制造商已经停止生产该型号，并通知我们，正常运行所需的关键部件的制造已经停止。考虑到仪器的使用年限，我们每6-8个月执行一次关键维护和部件更换是正常的，但到2018年8月将不再提供。因此，仪器的故障将有效地结束其使用，并使我的研究计划没有适合于生物测定引导的多肽定量纯化的系统，这是我的NSERC发现研究计划的基石。在加里堡校区或马尼托巴大学的医学院校区没有其他可用的Prep-Hplc系统。因此，这项提议的目的是购买一种新的Prep-HPLC系统，使我的发现计划能够继续创新地表征食品蛋白质衍生多肽的结构-功能特性。Prep-Hplc提供了最快速和最具成本效益的方法来分离和提纯数量足够大的生物分子，以便在多种体外分析和动物疾病模型中进行测试。这是因为慢性动物饲养实验最适合于确定生物活性化合物在预防或降低疾病病理强度方面的长期作用能力。预科-高效液相色谱法对于培养高素质人才也至关重要，包括1名博士后研究员和6名研究生，因为它能够提供高质量论文工作和及时毕业所需的分数。利用该仪器开发的技术将促进加拿大种植的作物或牲畜的增值利用，为加拿大经济带来显著回报。该仪器将用于促进研究生、博士后研究员和本科生在与维持体内平衡的预防性生物活性物质有关的食品科学和生命科学领域的跨学科培训。

Food protein-derived peptides remain a critical source of information for the discovery of fundamental scientific knowledge on relationships between the structure and function of bioactive agents. This is because of the almost non-exhaustive possible combinations of the 20 naturally-occurring amino acids within the context of peptide chain length (2-20 residues). However, due to the similarity in peptide size and chain length, the isolation, purification, functional characterization, and in vivo testing of bioactive peptides require quantitative analysis using high pressure liquid chromatography (HPLC) systems. At the Department of Food and Human Nutritional Sciences, University of Manitoba, the 10-yr old preparative HPLC (Prep-HPLC) system available for my NSERC peptide Discovery research will no longer be serviceable after July 2018. This is because the manufacturer has discontinued the model and given us notice that manufacture of critical parts needed for regular operation has been discontinued. Taking into consideration the instrument's age, it is normal for us to perform critical maintenance and parts replacements every 6-8 months but this will no longer be available by August 2018. Thus, a breakdown of the instrument will effectively end its use and leave my research program without a system suitable for bio-assay guided quantitative purification of peptides, which is the cornerstone of my NSERC Discovery research program. There is no other Prep-HPLC system available at the Fort Garry campus or the medical campus of the University of Manitoba. Therefore, the aim of this proposal is purchase a new Prep-HPLC system that will enable my Discovery program to continue innovative characterization of the structure-function properties of food protein-derived peptides. The Prep-HPLC provides the most rapid and cost-effective means of separating and purifying biological molecules in quantities that are large enough to permit testing in multiple in vitro assays and animal disease models. This is because chronic animal feeding experiments are the most suitable for determining the ability of bioactive compounds to act on a long-term basis in preventing or reducing the pathological intensity of diseases. The Prep-HPLC is also critical to training of Highly Qualified Personnel that includes one postdoctoral fellow and 6 graduate students because it enables provision of fractions in quantities that are required for high quality thesis work and timely graduation. Technology developed with the instrument will facilitate value-added utilization of Canadian-grown crops or livestock with significant returns to the Canadian economy. The instrument will be used to facilitate interdisciplinary training of graduate students, postdoctoral fellows and undergraduate students in the areas of food science and life sciences related to preventive bioactive agents for homeostasis maintenance.