A novel biofungicide derived from black soldier fly (Hermetia illucens) larval frass**********

消费者越来越多地寻求在尊重日益严格的环境准则的制度下种植的优质产品。各种来源的有机改良剂，如堆肥或粪肥，被认为是营养物质的良好来源，经常被用作施肥的来源。除了与使用有机改进剂有关的潜在环境效益外，它们还显示出控制土壤传播的植物病原体的能力。堆肥在抑制植物病害方面的有效性已在文献中有所描述。考虑到各种蔬菜和观赏植物容易受到多种病原体引起的真菌疾病的影响，使用有机材料的提取物可以防止这些疾病造成的损失。近年来，我们的研究小组开发了利用昆虫幼虫对有机废弃物进行生物转化的策略。这种方法将食物垃圾升级，以生产有价值的动物饲料原料。这一生物过程的二次产物是被称为“草”的幼虫粪便，除了富含宏观和微量元素外，它还富含有机物，这些元素已被证明是有价值的园艺作物土壤改进剂。这种生物资源也是独特微生物群落的丰富来源。我们实验室最近的研究表明，从我们的昆虫幼虫生物转化设施中提取的草明显抑制了一种经济上重要的植物真菌病原体——圆霉的生长。过滤灭菌的原始培养基导致消除这种影响，表明真菌生长抑制是一种微生物介导的现象。本研究旨在证明昆虫幼虫草对园艺作物病害抑制的影响，了解草特性对处理植物增强植物保护作用的影响。我们将采用下一代宏基因组测序来鉴定参与观察到的效应的微生物群落。这项工作将为开发新型生物杀菌剂铺平道路，以协助生产者追求更可持续的工业发展。

Consumers are increasingly seeking quality produce grown under systems respecting progressively more restrictive environmental guidelines. Organic amendments of diverse origin such as composts or manure are considered a good source of nutrients and are often used as a source of fertilization. In addition to the potential environmental benefits related to the use of organic amendments, they also have demonstrated an ability to control soil-borne plant pathogens. The effectiveness of composts in the suppression of plant diseases has been described in the literature. Considering that various vegetable and ornamental plants are susceptible to fungal diseases caused by a multitude of pathogens, the use of extracts from organic materials may prevent losses resulting from such diseases.Recent efforts by our research group have developed strategies for biotransforming waste organic residues using insect larvae. This approach upcycles food wastes to produce valuable animal feed ingredients. A secondary product of this bioprocess is larval excrement called 'frass', which is rich in organic matter in addition to maco and micro elements that have proven valuable as soil amendments for horticultural crops. This bioresource is also a rich source of unique microbial communities. Recent work from our lab has demonstrated that frass from our insect larvae bioconversion facility significantly inhibits the growth of Pythium ultimum, and economically-important plant fungal pathogen. Filter-sterilization of the frass media resulted in the elimination of this effect, demonstrating that of fungal growth inhibition is a microbial-mediated phenomenon. The current proposal seeks to demonstrate the impact of insect larval frass on disease suppressiveness in horticultural crops, to understand the impacts of frass characteristics on the augemented phytoprotective effects in treated plants. We will employ next-generation metagenomic sequencing to identify microbial communities involved in the observed effects. This work will pave the way for the development of novel biofungicides to assist producers to pursue enhanced sustainable industry development.