Antibacterial activity of silver nanoparticles towards oral and wound bacteria embedded in biofilms

治疗细菌感染的生物的部分困难在于细菌通常嵌入到生物膜中，生物膜是一种复杂的超结构，细菌微菌落分散在**细胞外聚合物、脂类和代谢物的基质中。为了准备有效的治疗方法，能够**有效地在复杂的生物膜基质中传输的药物和分子的开发是至关重要的。**鉴于生物膜的复杂性和多重耐药性的发展，非传统抗菌剂**在治疗病原微生物方面正在引起人们的兴趣。在这种背景下，SK Nano正在**生产新型纳米材料，作为传统抗菌药物的低成本替代品。纳米粒子在抗菌产品中有许多预计的应用，这使它们在伤口护理、皮肤病、兽医疾病和口腔健康问题方面具有巨大的商业化潜力。使用我们实验室提供的**最先进的技术(单粒子电感耦合等离子体光谱，**SP-ICPMS；动态光散射，DLS；分析超速离心法，AUC和透射电子显微镜**)，我们将首先精确地表征SK Nano正在合成的不同尺寸、形状和**表面涂层的新型银纳米材料。在第二步中，将使用共聚焦扫描激光显微镜(CSLM)对AgNPs(三种**不同尺寸、三种不同形式、三种不同表面涂层)的抗菌性能进行量化。纳米粒子在细菌生物膜中的扩散将通过荧光相关光谱进行**评估。该项目将使我们能够更好地了解**将新材料的性质与其生物功能联系起来的结构-功能关系。**这些信息将加深我们对这些产品的机理理解，并使SK Nano**能够**更好地设计具有适当抗菌活性的未来产品。

Part of the difficulty in treating organisms for bacterial infection is that the bacteria are often embedded within**biofilms, which are complex superstructures in which bacterial microcolonies are dispersed in a matrix of**extracellular polymeric substances, lipids, and metabolites. The development of drugs and molecules that can**efficiently be transported across the complex biofilm matrix is essential in order to prepare effect treatments.**Given the complexity of the biofilms and the development of multidrug resistance, non-traditional antibacterial**agents are of emerging interest for the treatment of pathogenic microorganisms. In this context, SK Nano are**producing novel nanomaterials as a cost-effective alternative to the traditional anti-bacterials. The**nanoparticles have numerous projected applications in antibacterial products, which gives them great potential**for commercialization related to wound care, dermatology, veterinary diseases and oral health problems. Using**state-of-the art techniques that are available in our lab (single particle inductively coupled plasma spectroscopy,**SP-ICPMS; dynamic light scattering, DLS; analytical ultracentrifugation, AUC and transmission electron**microscopy, TEM), we will first precisely characterize novel Ag nanomaterials with different sizes, shapes and**surface coatings are being synthesized by SK Nano. In a second step, anti-bacterial properties of AgNPs (three**different sizes, three different forms, three different surface coatings) will be quantified using confocal**scanning laser microscopy (CSLM). Diffusion of the nanoparticles within the bacterial biofilms will be**evaluated by fluorescence correlation spectroscopy. The project will enable us to better understand**structure-function relationships relating the properties of the novel materials with their biological function.**Such information will both further our mechanistic understanding of these products and allow SK Nano to**better design future products with appropriate antibacterial activity.