Surveillance for Tenacibaculum sp. the cause of mouth rot in Atlantic salmon raised by MOWI West Canada

西加拿大农业部在不列颠哥伦比亚省可持续地饲养大西洋鲑鱼。由网纹夜蛾引起的鼠疫是影响网箱青枯病的重要产物性病害。损失是由于死亡造成的，但更重要的是由于增长放缓。受影响鱼类的治疗费用很高，而且往往不得不重复治疗，从而产生巨大的成本。与这种疾病相关的细菌也可能对现有的抗菌剂产生抗药性。因此，除抗菌治疗之外的管理策略是至关重要的。这项提议将使用根据NSERCEngage提议开发的定量聚合酶链式反应(QPCR)分析来量化责任细菌。了解鱼、环境和水中有机体的藏身之处，以及了解暴发前、暴发期间和暴发后何时出现高细菌负荷，是更好地管理暴发所需的关键信息。这些检测还可以用来评估抗菌治疗的效果，并了解为什么它们经常不成功。这些检测方法将允许改进MHC网络点的鼠喉主动管理，并应允许除抗菌治疗外的其他选择或减少抗菌药物使用的需要。

MOWI West Canada sustainably raises Atlantic salmon in British Columbia. An important productiondisease affecting smolts in netpens is mouthrot caused by Tenacibaculum sp. Losses occur due to mortality butas importantly due to reduced growth. Treatment of affected fish is expensive and often has to be repeatedincurring significant costs. The bacteria associated with this disease can also be resistant to the availableantimicrobial agents. Management strategies other than antimicrobial therapy are therefore of critical need.This proposal will use quantitative polymerase chain reaction (qPCR) assays developed under a NSERCEngage proposal for quantification of the responsible bacteria. Understanding where the organism is harbouredin the fish, environment and water and knowing when high bacterial loads are present before, during and afteran outbreak are key pieces of information needed to better manage outbreaks. The assays can also be used toevaluate the efficacy of antimicrobial treatments and understand why they are often not successful. Theseassays will allow improvements to proactive management of mouthrot at MHC netpen sites and should allowoptions other than antimicrobial therapy or that reduce the need for antimicrobial use.