A Preparative HPLC Instrument for the Purification of Small Molecules

制备型高压液体净化（HPLC）系统用于有机分子的净化。制备型高效液相色谱是最强大的纯化方法之一，适用于数百毫克的分子纯化。******目前合成有机化学家的最佳实践要求纯化纯度为95%的产品。该系统将允许纯化各种不同的分子类型，包括杂环、生物碱、多肽、沉积肽、大环、萜类、肽拟物、肽小分子和其他生物偶联物。该系统将允许快速纯化具有挑战性的样品，否则将不可能或非常难以纯化使用传统方法，如闪柱色谱法。此外，高效液相色谱法纯化通常需要作为生物测试前的最后一步。提议的高效液相色谱系统将对我们解决合成和药物化学问题和项目的能力产生变革性影响，否则这些问题和项目是不可能实现的。******提议的高效液相色谱系统将被用作“跑龙套”仪器，主要服务于两个研究小组的学生和博士后研究人员的需求。然而，该系统将提供给多伦多大学化学系的其他用户。在有机化学领域受过训练的学生受到制药、生物技术和材料行业雇主的高度追捧。因此，这台主力仪器将为HQP的合成有机或药物化学培训提供宝贵的培训经验

A preparative high-pressure liquid purification (HPLC) system is requested for the purification of organic molecules. Preparative HPLC is one of the most powerful purification methods, and is amenable to the purification of molecules on scales up to hundreds of milligrams.******Current best practice for synthetic organic chemists requires purification of products with purities of 95%. The proposed system will allow for the purification of a variety of different molecular types, including heterocycles, alkaloids, peptides, depsipeptides, macrocycles, terpenoids, peptidomimetics, and peptide small-molecule and other bioconjugates. The system will allow for rapid purification of challenging samples that would otherwise be impossible or very difficult to purify using conventional methods such as flash column chromatography. Moreover, HPLC purification is often required as a final step before biological testing. The proposed HPLC system will have a transformative effect on our ability to tackle synthetic and medicinal chemistry problems and projects that would otherwise not be possible.******The proposed HPLC system will be utilized as a “walk-on” instrument and will primarily service the needs of students and post-doctoral researchers from two research groups. However, the system will be made available to other users in the Department of Chemistry at the University of Toronto. Students trained in the area of organic chemistry are highly sought after by employers in the pharmaceutical, biotech, and materials sectors. As such, this workhorse instrument will provide invaluable training experience for HQP with synthetic organic or medicinal chemistry training.**