Upgrades to Multi-Lab Gas Chromatography System

气相色谱(GC)是测量生物样品中脂肪酸和其他脂质的行业标准方法。由于脂肪酸和脂类参与了所有生物的许多细胞过程，正确理解这些过程需要从实验样本中进行准确的测量。GC是执行这些分析测量的最准确和最具成本效益的方法。*我们正在申请资金，以对我们全面的、多实验室的GC计划进行重大升级。这包括(1)安捷伦8890 GC系统、(2)安捷伦7693自动进样器和(3)Thermo TriPlus 100LS自动进样器。这些自动取样器将是安装在现有GC上的替代品。脂质组学核心设施得到医学和牙科学院、妇女和儿童健康研究所和艾伯塔大学的高度补贴，为各院系的大学研究人员提供负担得起的脂质分析服务。申请的资金将为这一倡议提供强有力的支持，不仅使3名申请者受益，还将使许多其他研究血脂的研究人员受益。*申请者共享GC基础设施的协作性是一种独特的、高度功能性的安排，直接和间接参与的各方都能受益。利用这种双通道GC，将field博士实验室的脂肪酸分析吞吐量增加一倍，将提高包括Lehner博士和Proctor博士在内的核心设备用户的质量并缩短等待时间。这反过来又将使该机制能够进一步发展和扩大所提供的分析方法。新的8890 GC将由学员和HQP在菲尔德博士的实验室一周7天每天24小时使用，修复后的GC将由来自大学各地的研究人员每天使用。更广泛地说，气相色谱是一项重要的、在学术界、政府和工业中广泛使用的技术。除了用于测量血脂和其他基础研究应用外，GC还广泛用于石油、替代能源、食品和香料、化妆品、医疗保健、制药和环境行业的研究和开发。学习操作和分析来自GC仪器的数据的实习生获得了对他们未来职业生涯非常有价值的技能。通过在研究环境中使用GC获得的深入知识对于培养高素质的人员(HQP)来说是非常宝贵的，这些人员将在学术界、政府、行业和更多领域进入富有成效的职业生涯。

Gas chromatography (GC) is the industry-standard method for measuring fatty acids and other lipids in biological samples. Because fatty acids and lipids are involved in many cellular processes in all living things, proper understanding of these processes requires accurate measurements from experimental samples. GC is the most accurate and cost-effective way of performing these analytical measurements.******We are requesting funding for a major upgrade to our comprehensive, multi-lab GC program. This includes (1) an Agilent 8890 GC System, (2) an Agilent 7693 autosampler, and (3) a Thermo TriPlus 100LS autosampler. These autosamplers will be replacements installed on existing GCs. The Lipidomics Core Facility is highly subsidized by the Faculty of Medicine and Dentistry, the Women and Children's Health Research Institute, and the University of Alberta, and provides affordable lipid analytical services to university researchers across faculties. The requested funding will provide strong support to this initiative, benefitting not only the 3 applicants, but also many other investigators who study lipids. ******The collaborative nature of the applicants' shared GC infrastructure is a unique and highly-functional arrangement that benefits all parties who are directly and indirectly involved. Doubling the throughput of fatty acid analysis in the lab of Dr. Field with this dual-channel GC will lead to improvements in quality and shorter wait times for Core Facility users including Drs. Lehner and Proctor. This in-turn will allow the Facility to further develop and expand the analytical methodology offered. The new 8890 GC will be used by trainees and HQP 24 hours a day, 7 days a week in the lab of Dr. Field, and the repaired GCs will be used daily by researchers from across the University. ******More broadly, gas chromatography is an important and heavily-used technique in academia, government, and industry. In addition to their use in measuring lipids and other basic research applications, GCs are widely used for research and development in the petroleum, alternative energy, foods and flavours, cosmetics, healthcare, pharmaceutical, and environmental industries. Trainees who learn to operate and analyze data from GC instruments gain skills that are incredibly valuable for their future careers. The in-depth knowledge gained by using GCs in a research setting is invaluable in producing highly-qualified personnel (HQP) who progress into productive careers in academia, government, industry, and more.*****