COVID-19 and targeting SARS-CoV-2 virus: Direct and RT-PCR sequences from wastewater collected from treatment plants (Sudbury - Ontario)

我们需要能够迅速和有效地确定严重急性呼吸综合征冠状病毒2(SARS-CoV-2)在我们社区的存在和流行情况，以及这种存在对社会和医疗干预措施的反应。 在许多方面，我们城市的未经处理的污水是一个奇妙的基因金矿，特别是对收集在那里的病原体来说。我们正计划挖掘这一遗传资源，以使用一种新的基因测序技术来检测和评估SARS-CoV-2的存在。这项新技术可以直接应用于病毒基因组测序，也可以使用不同的方法通过特定的可变区对病毒基因组进行排序。我们建议使用这两种方法来确定和量化污水收集区大流行病毒的存在。 这些信息将被用来估计病毒的传播，并跟踪疫情随时间的变化。这些结果将有助于地方政府做出知情的、以证据为基础的决策，以帮助减缓和控制疫情。一旦协议和关系建立良好，我们将能够快速扩展该项目，并将其扩展到不同的地理区域和人口。该项目是北方健康科学公司、劳伦斯大学和大萨德伯里市为抗击大流行而建立的合作伙伴关系的结果。

We need to be able to rapidly and efficiently determine the presence and prevalence of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in our communities and how this presence responds to the social and medical interventions. In many ways, the raw sewage of our cities is a fantastic genetic gold mine, particularly for the pathogens that collect there. We are planning to mine this genetic resource to detect and evaluate the presence of SARS-CoV-2 using a new genetic sequencing technology. This new technology can be applied to sequence the virus genome either directly or through specific variable genetic regions using different approaches. We propose to use these two methodologies to identify and quantify the presence of the pandemic virus in the sewage collection areas. This information will be used as to estimate the virus' spread and to following changes in the outbreak through time. These results will aid local government in making informed, evidence-based, decisions to help slow and control of the pandemic. Once the protocols and relationships are well established, we will be able to scale this project quickly and extend it to different geographic areas and populations. This project is the result of a partnership between Health Sciences North, Laurentian University and the City of Greater Sudbury established to combat the pandemic.