Tracing element pathways and processes of Ca, Fe, Cu, and Zn in the geosphere and biosphere: Precision measurement with isotopic tools

我的研究小组的重点是开发高精度的金属同位素丰度测量技术及其应用，以解释这些数据的金属过程和金属在生命系统中的途径。 我们看到短期的影响，在推进我们的理解如何通过生物化学过程的金属相互作用。 正如最近与医学同事的合作实验中成功证明的那样，该实验探索了抗生素治疗影响的铜同化的变化（米勒等人，2019年），同位素工具有可能揭示隐藏在更传统的分析方法中的生化途径。 从长远来看，我们相信我们的研究是变革性的，我们的愿景是同位素丰度工具成为维护健康和健康的标准和重要组成部分。 这项研究计划是朝着这一方向迈出的关键的基础性一步。 近期，该计划的目标是开发和部署稳定同位素技术，以揭示地球化学系统中Ca，Fe，Cu和Zn同位素丰度的分布和变化。 我们试图确定关键的相互作用，同位素组成发生变化，并了解负责同位素变异的物理化学过程。 这一项目的目标是通过发展在气象学上可靠的分析技术来测量同位素组成，以便提取可靠的信息。结果被放置在基于计算模型的理论背景下，以获得对结果的有意义的解释。 研究计划的基本性质和跨学科受众反映在我们出版的期刊以及本DG申请所附参考文献中引用的期刊中。 过去五年取得了重大进展，拟议的研究是这一成功研究计划的延续。 亮点包括吸引了一群有能力的HQP到研究小组（我的第一个博士生毕业，第一个博士后学者加入了小组，国际博士生访问实验室）。 我们在美国国家科学院院刊、金属组学和分析原子光谱学杂志等领先期刊上发表了我们的工作。我们开始与健康科学（K。Sharkey，Cumming医学院，UofC），生物科学（V. Zaremberg和H。Vogel，UofC），Marine Biogeochemistry（A.鲍伊和B。Proemse，University of Tasmania，Australia）和化学（J. Gailer，UofC; T. Prohaska和J. Irrgeher，Montanuniversität，Leoben，奥地利）。最后，我们从各种来源吸引了新的资金来源。

The focus of my Research Group is the development of high precision metal isotope abundance measurement techniques and their application to interpreting these data in the context of metal processes and pathways of metals in living systems. We see short term impacts in advancing our understanding of how metals interact through biochemical processes. As successfully demonstrated in a recent collaborative experiment with colleagues in Medicine exploring changes in copper assimilation affected by antibiotic treatment (Miller et al., 2019), isotopic tools have the potential to reveal biochemical pathways that are hidden from more traditional analytical approaches. In the long term, we believe our research is transformative and our vision is that isotope abundance tools become a standard and vital component in maintaining health and wellness. This research program is the critical fundamental step in this direction. In the near term, the goals of the program are to develop and deploy stable isotope techniques to uncover the distribution and variability of isotopic abundances of Ca, Fe, Cu, and Zn in biogeochemical systems. We seek to identify critical interactions where changes in isotopic composition occur and to understand the physiochemical processes responsible for isotopic variability. The objectives of this project are realized through development of meteorologically sound analytical techniques to measure isotopic composition such that one extracts reliable information. Results are placed within a theoretical context based on computational models to derive a meaningful interpretation of the results. The fundamental nature and the interdisciplinary audience of the research program are reflected in the journals where we publish as well as the journals cited in the References attached to this DG application. The past five years saw significant progress and the proposed research is a continuation of this successful research program. Highlights include attracting a diverse group of capable HQP to the research group (my first PhD students graduate, first postdoctoral scholar joined the group, international PhD students visiting the lab). We published our work in leading journals such as Proceedings of the National Academy of Sciences, Metallomics, and the Journal of Analytical Atomic Spectrometry. We embarked on productive collaborations with colleagues in Health Sciences (K. Sharkey, Cumming School of Medicine, UofC), Bioscience (V. Zaremberg and H. Vogel, UofC), Marine Biogeochemistry (A. Bowie and B. Proemse, University of Tasmania, Australia) and Chemistry (J. Gailer, UofC; T. Prohaska and J. Irrgeher, Montanuniversität, Leoben, Austria). Finally, we have attracted new sources of funding from diverse sources.