Multi-mode microplate reader

多模式酶标仪是一种使用多种不同分析技术检测细胞内或细胞和组织提取物中的生化事件的仪器。它依赖于使用不同波长的光吸收、荧光和发光特性，使用96孔板以微型形式检测这些生化事件。虽然简单的吸光度酶标仪是常用的，但在本申请中，我要求资金在约克大学校园内购买第一个此类设备系统：一个多模式系统，可以使用吸光度，荧光和发光来获取数据。 这个系统也将取代我的非常旧的，过时的系统，这些系统在过去很好地为我们服务，但已经变得非常不可靠，或者具有不利于更高吞吐量应用的单管操作能力。我的学员和肌肉健康研究中心（MHRC）的同事将使用这个新系统来分析1）收缩活动激活的基因转录（即运动），2）来自线粒体的活性氧物质的释放，3）从用线粒体生物发生诱导剂处理的肌细胞获得的氧消耗数据，4）肌管中的总蛋白质和核丰度，和5）肌细胞中的心磷脂浓度。所有这些措施都直接支持我们的NSERC发现赠款目标。购置这台新设备对我们实现NSERC DG研究目标至关重要。延迟收购将对我们的进展产生重大影响，因为我们每天都在使用酶标仪技术。 这些设备也将对人力资源规划人员的培训产生重大影响。我的实验室对硕士，博士和博士后的机会需求很大，目前我每年有10名学员。酶标仪将使我能够在市场上培训这些HQP，先进和最新的技术（吸光度，荧光，发光），这将在未来的学术，临床和其他研究和分析环境中为他们提供良好的服务。 此外，作为MHRC的主任，我还提交了这份申请，以帮助支持生物系，运动机能学和健康科学学院以及MHRC的其他25名教师，目的是提高我们目前非常有限的使用这些重要的生化分析能力，约克大学的先进方法。

A multi-mode microplate reader is an instrument that uses several different analytical techniques to detect biochemical events within cells, or in extracts from cells and tissues. It relies on the use of light absorbance, fluorescence and luminescence properties which use different wavelengths to detect these biochemical events in a miniature format using 96 well plates. While simple absorbance microplate readers are in common use, in this application I am requesting funds to acquire the first equipment system of this kind on the York University campus: a multi-modal system that can use absorbance, fluorescence as well as luminescence to acquire data. This system will also replace my very old, obsolete systems which served us well in the past, but that have become extremely unreliable, or have single tube operation capabilities which are not conducive to higher throughput applications. My trainees and Muscle Health Research Centre (MHRC) colleagues will use this new system to analyze 1) the transcription of genes that are activated by contractile activity (i.e. exercise), 2) the emission of reactive oxygen species from mitochondria, 3) oxygen consumption data acquired from muscle cells treated with mitochondrial biogenesis-inducing agents, 4) total protein and nuclear abundance in myotubes, and 5) cardiolipin concentration in muscle cells, among many other applications. All of these measures directly support our NSERC Discovery grant objectives. Acquisition of this new equipment is absolutely essential for us to progress in achieving our NSERC DG research goals. A delay in its acquisition will have a significant impact on our progress, since we use microplate reader technology every day. This equipment will also have a significant impact on the training of HQP. My laboratory is in high demand for MSc, PhD and post-doctoral opportunities, and I currently have 10 trainees on a yearly basis. The microplate reader will allow me to train these HQP in marketable, sophisticated and up-to-date technology (absorbance, fluorescence, luminescence), which will serve them well in academic, clinical and other research and analysis settings in the future. In addition, as the Director of the MHRC, I am also submitting this application to help support the >25 other faculty members in the Department of Biology, the School of Kinesiology and Health Science, and the MHRC, with the goal of increasing our currently very limited capacity for biochemical analyses using these important, advanced methodologies at York University.