Multi-mode microplate reader

多模式微板阅读器是一种使用几种不同的分析技术来检测细胞内或细胞和组织提取物中的生化事件的仪器。它依赖于光吸收、荧光和发光特性的使用，这些特性使用不同的波长来检测使用96个孔板的微型格式的这些生化事件。虽然简单的吸光度微板阅读器是常用的，但在这项申请中，我正在申请资金，以购买约克大学校园内第一个此类设备系统：一个可以使用吸光度、荧光和发光来获取数据的多模式系统。这个系统也将取代我的非常旧的、过时的系统，这些系统过去运行得很好，但已经变得非常不可靠，或者具有单管运行能力，不利于更高吞吐量的应用。我的受训人员和肌肉健康研究中心(MHRC)的同事将使用这个新系统来分析1)收缩活动(即运动)激活的基因的转录，2)线粒体活性氧物种的释放，3)用线粒体生物发生诱导剂处理的肌肉细胞的耗氧量数据，4)肌管中的总蛋白质和核丰度，以及5)肌肉细胞中心磷脂的浓度，以及许多其他应用。所有这些措施都直接支持我们的NSERC发现拨款目标。获得这种新设备对于我们实现NSERC DG研究目标是绝对必要的。推迟收购将对我们的进展产生重大影响，因为我们每天都在使用微板阅读器技术。该设备还将对HQP的培训产生重大影响。我的实验室非常需要硕士、博士和博士后机会，目前我每年有10名实习生。微板阅读器将允许我在市场上对这些HQP进行培训，使他们掌握先进的最新技术(吸光度、荧光、发光)，这些技术将在未来的学术、临床和其他研究和分析环境中很好地服务于他们。 此外，作为MHRC的主任，我也提交了这份申请，以帮助支持生物系、运动学与健康科学学院和MHRC的其他25名教职员工，目标是提高我们目前非常有限的能力，使用约克大学这些重要的、先进的方法进行生化分析。

A multi-mode microplate reader is an instrument that uses several different analytical techniques to detect biochemical events within cells, or in extracts from cells and tissues. It relies on the use of light absorbance, fluorescence and luminescence properties which use different wavelengths to detect these biochemical events in a miniature format using 96 well plates. While simple absorbance microplate readers are in common use, in this application I am requesting funds to acquire the first equipment system of this kind on the York University campus: a multi-modal system that can use absorbance, fluorescence as well as luminescence to acquire data. This system will also replace my very old, obsolete systems which served us well in the past, but that have become extremely unreliable, or have single tube operation capabilities which are not conducive to higher throughput applications. My trainees and Muscle Health Research Centre (MHRC) colleagues will use this new system to analyze 1) the transcription of genes that are activated by contractile activity (i.e. exercise), 2) the emission of reactive oxygen species from mitochondria, 3) oxygen consumption data acquired from muscle cells treated with mitochondrial biogenesis-inducing agents, 4) total protein and nuclear abundance in myotubes, and 5) cardiolipin concentration in muscle cells, among many other applications. All of these measures directly support our NSERC Discovery grant objectives. Acquisition of this new equipment is absolutely essential for us to progress in achieving our NSERC DG research goals. A delay in its acquisition will have a significant impact on our progress, since we use microplate reader technology every day. This equipment will also have a significant impact on the training of HQP. My laboratory is in high demand for MSc, PhD and post-doctoral opportunities, and I currently have 10 trainees on a yearly basis. The microplate reader will allow me to train these HQP in marketable, sophisticated and up-to-date technology (absorbance, fluorescence, luminescence), which will serve them well in academic, clinical and other research and analysis settings in the future. In addition, as the Director of the MHRC, I am also submitting this application to help support the >25 other faculty members in the Department of Biology, the School of Kinesiology and Health Science, and the MHRC, with the goal of increasing our currently very limited capacity for biochemical analyses using these important, advanced methodologies at York University.