Novel silver nanoparticles as antibacterial agents

我们的目标是扩大低成本技术的优势，用于环境友好型杀菌银纳米颗粒(Ag NPs)的合成。AG NPs具有独特的性能，专门针对医疗保健领域的特定问题而设计，将使用不同类型的植物提取物、精油和天然抗菌剂来生产。我们的合作伙伴开发的独特的合成方案可以产生不同尺寸和形貌的银(0)纳米颗粒。目标是确定在天然抗菌涂层和银纳米颗粒之间产生最佳协同效应的条件，目标是开发具有消除耐药细菌的独特能力的产品。我们将通过测试这些产品对几种生物被膜形成细菌的杀灭能力来研究它们的抗菌效果，这些细菌包括：变形链球菌、大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌。我们的目标是详细了解Ag NP对细菌的复杂作用，长期目标是将它们整合到水凝胶支架中，用于治疗慢性病和组织再生。利用动态光散射(DLS)、透射电子显微镜(TEM)、单粒子电感耦合等离子体质谱(SP-ICPMS)和单细胞电感耦合等离子体质谱(SC-ICPMS)对纳米粒子进行了严格的表征。

We aim to extend the advantages of a low-cost technique for the environmentally friendly synthesis of bactericidal silver nanoparticles (Ag NPs). Ag NPs, with unique properties, designed deliberately for specific problems in the health care field will be produced using different types of plant extracts, essential oils and natural antibacterials. The unique synthesis protocol developed by our partner produces different sizes and morphologies of Ag(0) nanoparticles. The goal is to identify the conditions that will give the best synergies between the natural antibacterial coatings and silver nanoparticles, with the goal of developing products that have a unique capacity to eradicate drug resistant bacteria. We will investigate the antibacterial efficacy of these products, by testing their ability to kill several biofilm forming bacterial strains including: Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. We aim to gain detailed insight into the complex action of Ag NP on the bacteria, with the long-term goal of incorporating them into hydrogel scaffolds for the treatment of chronic diseases and tissue regeneration. Nanoparticles will be rigorously characterized using Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), Single Particle Inductively Coupled Plasma Mass Spectroscopy (SP-ICP-MS) and Single Cell Inductively Coupled Plasma Mass Spectroscopy (SC-ICP-MS).