Design of a new PLLA vascular scaffold coated with stem cell-derived nanovesicles for critical limb ischemia

血管支架在重度肢体缺血（CLI）的治疗中得到了广泛的研究。然而，术后再狭窄的风险增加了调节炎症或内膜生长的其他因素的需求。采用生物可吸收支架（生物可吸收支架）和干细胞来源的纳米囊泡作为药物递送涂层的机械支持联合治疗，可获得更好的患者预后和更快的恢复。在本研究中，主要目的是设计、开发和测试一种基于干细胞源性纳米囊泡的新型生物可吸收支架涂层支架，以提高生物相容性和增强治疗效果。将生成支架几何形状并进行数值模拟，以评估部署和药物分散动力学。最佳候选者将用激光微加工进行原型制作。在与合作伙伴的合作下，这些原型将被纳米粒子技术所覆盖。随后，涂层原型的药物分散动力学将使用专用液压装置进行测试，再现解剖结构和生理流动条件。合作伙伴将提供设计标准，并通过3D OCT成像（用于支架部署评估）验证设计性能，并协助进行激光原型设计和机械性能测试。

Vascular scaffolds have been widely studied for the treatment of critical limb ischemia (CLI). However, the risk of postoperative restenosis raises the need of other factors that regulate inflammation or intimal growth. A combination therapy with mechanical support using a bioresorbable scaffold (bioresorbable stent) and biochemical cues from stem cell-derived nanovesicles as a coating for drug deliver is put forward for better patient outcomes and faster recoveries.In this study, the main objective is to design, develop and test a new bioresorbable scaffold coated stent based on stem cell-derived nanovesicles for higher biocompatibility and enhanced therapeutic efficacy. Stent geometries will be generated and modelled numerically for assessing deployment and drug dispersion kinetics. The best candidates will be prototyped with laser micro-machining. In collaboration with the partner, the prototypes will be coated with the nano-seicles technology. Subsequently, the drug dispersion kinetics of the coated prototypes will tested using a dedicated hydraulic setup reproducing the anatomical structures and physiological flow conditions. The partner, will provide the design criteria and verify the design performance with 3D OCT imaging (for scaffold deployment assessment) and assist for the laser prototyping and mechanical performance testing.