Tools for molecular physiology research

此RTI应用程序用于在班菲尔德海洋科学中心进行分子生理学研究所必需的一套相互关联的工具，班菲尔德海洋科学中心是位于不列颠哥伦比亚省温哥华岛西海岸的世界著名海洋研究站。我们的集体研究集中在理解生理系统中的进化关系。我们的研究通过专注于基本分子生理学和人类对鱼类造成的损害的影响的调查，为海洋生态系统的保护和保全做出了贡献。这项研究的性质要求持续接触这些海洋环境中的水生生物自然种群。我们研究地点的偏远性质与不断增长的基因组学领域有关，因此有必要在班菲尔德海洋科学中心建立一个分子套件。这将提供必要的工具，将基因组学整合到我们的分子生理学计划中。我们正在申请资金，为班菲尔德海洋科学中心的研究购买一套分子套件，以支持我们的分子生理学研究计划。这一要求包括一个新的液体闪烁计数器，这是开展基于放射性示踪剂的实验以了解海洋鱼类离子传输的细胞和分子机制所必需的；一个新的超低温冷冻机(-80摄氏度)，用于安全储存和保存生物样品；以及一台纳米液滴分光光度计和坚固耐用的聚合酶链式反应机器，它将能够立即分离核糖核酸和基因合成，以便随后进行基因表达分析。每一台设备都是一个相互关联的设备组的一部分，这些设备将支持多个由NSERC资助的研究项目，从而允许每个共同申请者以及许多其他NSERC和非NSERC资助的研究人员继续推进研究，并为班菲尔德海洋科学中心(BMSC)的HQP提供出色的指导。

This RTI application is for a suite of inter-related tools essential for conducting molecular physiology research at Bamfield Marine Sciences Centre, a world-renowned marine research station on the West Coast of Vancouver Island BC. Our collective research is focused on understanding the evolutionary relationships in physiological systems. Our research contributes to the protection and preservation of ecosystems in oceans and seas by focusing on both fundamental molecular physiology and investigation of the effect of human-induced damage to fishes. The nature of this research requires continual access to natural populations of aquatic organisms in these marine environments. The remote nature of our research sites in association with the growing field of genomics necessitates a molecular suite be established at Bamfield Marine Sciences Centre. This will provides the necessary tools to integrate genomics into our molecular physiology programs. We are requesting funds to purchase a molecular suite for research at the Bamfield Marine Sciences Centre in support of our research programs in molecular physiology. This request includes a new liquid scintillation counter necessary for conducting radiotracer-based experiments to understand the cellular and molecular mechanisms of ion transport in marine fishes, a new ultra-low temperature freezer (-80 °C) s required for safe storage and preservation of biological samples, and both a nanodrop spectrophotometer and robust PCR machine that will allow for immediate isolation of RNA and cDNA synthesis for subsequent analysis of gene expression. Each piece of equipment forms part of an interrelated group of equipment that will support multiple NSERC-funded research programs thereby allowing each of the co-applicants plus numerous other NSERC and non-NSERC funded researchers who routinely use BMSC as part of their research to continue advancing research and providing outstanding mentoring of HQP at Bamfield Marine Sciences Centre (BMSC).