粪肠球菌穿越BeWo细胞/胎盘屏障的分子机制研究及功能评价

微生物与其生存环境（宿主）相互作用的研究是当今微生物学领域的热点与难点。本项目前期研究表明，定植于孕鼠肠道的肠球菌可以穿越肠道和胎盘屏障，移位至胎盘和仔鼠，然而其相关分子作用机制并不明确。迄今，肠球菌被科学界认为兼具益生和致病双重特性，备受争议，缺乏足够的科学依据，特别是其穿越对母胎的生物学作用文献鲜见，且无定论。本项目拟建立胎盘屏障的BeWo细胞模型和孕鼠动物模型，应用基因组学和蛋白组学的方法，结合激光共聚焦、基因芯片和蛋白质双向电泳、荧光定量PCR、免疫组化和基因敲除等技术，多角度多层次地观察肠球菌穿越对胎盘屏障结构、功能以及母胎的生物学作用，并从细胞和分子水平探讨肠球菌穿越的相关机制，为在食品和药品中合理控制使用肠球菌，丰富临床微生态学的基础理论提供科学数据

Bacteria-host cross-talking and interaction is a hard and hot-point in current microbiology research. In the previous study of our laboratory, Enterococcus faecalis, colonized in the intestinal tracts of pregnant mice, could translocation across intestinal and placental barriers, and thus reach fetus of mice. However, the molecular mechanism is not clear. Up-to-date, Enterococcus .spp attracted much disputation on its probiotic properties and pathogenic properties, due to the rare experimental data and literatures, especially, there is rare information on its translocation from mother to fetus. Hence, the biological function and molecular mechanism for translocation of Enterococcus .ssp across placental barrier is not yet well documented. In this project, we try to appraoch and summarize the translocation phenomenon of Enterococcus by setting up placental barriers i.e. BeWo cell line and pregnant mice, applying laser scanning confocal microscope, genomics and proteomic approaches, real-time fluorescence quantitative PCR and immunohistochemistry, in order to clarify the biological function and molecular mechanism for translocation of Enterococcus .ssp across placental barrier. The research can set up the foundation to reasonable control of the Enterococcus .ssp in food and Drug, and expand the basic theory of clinical micro-ecology