Molecular Basis of Regulatory Idiotopes

调节性独特位素的分子基础

基本信息

  • 批准号:
    8709711
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    1988
  • 资助国家:
    美国
  • 起止时间:
    1988-01-01 至 1989-06-30
  • 项目状态:
    已结题

项目摘要

Previous studies have led to the definition of a category of idiotope designated regulatory idiotopes which have been proposed to play an important role in idiotype-determined regulation of the immune response. High connectivity between B cell clones with various antigen specificities has been demonstrated, and such clones can be activated by antibodies specific for regulatory idiotopes. This phenomenon has been termed "cross-regulation". Furthermore, the majority of monoclonal antibodies expressing A48 regulatory idiotopes utilized V genes derived from the VH441-4 and VK10 families. Further studies will be aimed at exploiting these observations to gain further insight into the cellular and molecular mechanisms governing the expression of regulatory idiotopes. A rabbit antibody specific for a synthetic peptide corresponding to CDR1 of the heavy chain of A48 antibody and hybridomas from non- immunized mice selected with a VH441 germline gene probe have been prepared. The antibody to the synthetic peptide will be used to determine whether or not the antibodies sharing regulatory idiotopes express the same CDR1 sequence as the germline gene from which the A48 protein is derived. The antibodies produced by the hybridomas selected from non-immunized mice ("steady state") will be characterized with respect to their VH and VK families, their idiotypy, and their fine specificities. This should make it possible to determine the frequency of regulatory idiotopes in the steady state. The sequence of VH and VK genes encoding antibodies expressing regulatory idiotopes will be carried out and should enhance our understanding of the molecular factors contributing to the expression of regulatory idiotopes. The overall goal of these studies is to elucidate the immunochemical and molecular basis of regulatory iditopes. This knowledge should add to our understanding of the mechanisms by which immune responses are regulated.
以前的研究已经导致一类独特表位的定义指定的调节独特表位已被提出发挥重要作用的独特型确定的免疫反应的调节。已经证明了具有各种抗原特异性的B细胞克隆之间的高度连接性,并且这些克隆可以被对调节独特表位特异性的抗体激活。这种现象被称为“交叉调节”。此外,大多数表达A48调节独特位的单克隆抗体利用来自VH 441 -4和VK 10家族的V基因。进一步的研究将旨在利用这些观察结果,以进一步了解调控独特位表达的细胞和分子机制。制备了对对应于A48抗体重链CDR 1的合成肽具有特异性的兔抗体和来自用VH 441生殖系基因探针选择的非免疫小鼠的杂交瘤。合成肽的抗体将用于确定共享调节独特表位的抗体是否表达与A48蛋白质所来源的种系基因相同的CDR 1序列。由选自未免疫小鼠的杂交瘤产生的抗体(“稳态”)将在其VH和VK家族、其独特型和其精细特异性方面进行表征。这应该可以确定稳定状态下调控独特位的频率。将进行编码表达调控独特表位的抗体的VH和VK基因的测序,并应增强我们对促进调控独特表位表达的分子因素的理解。这些研究的总体目标是阐明调节iditopes的免疫化学和分子基础。这些知识应该有助于我们理解免疫反应的调节机制。

项目成果

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Constantin Bona其他文献

Constantin Bona的其他文献

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{{ truncateString('Constantin Bona', 18)}}的其他基金

Conference: Molecular Basis of the Immune Response, to be held on January 11, 12, 13, 1988, in New York City, NY.
会议:免疫反应的分子基础,将于 1988 年 1 月 11、12、13 日在纽约州纽约市举行。
  • 批准号:
    8713251
  • 财政年份:
    1987
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
Cellular and Molecular Basis of Regulatory Idiotypic Network
调节独特型网络的细胞和分子基础
  • 批准号:
    8408660
  • 财政年份:
    1984
  • 资助金额:
    --
  • 项目类别:
    Continuing grant
Conference on Immune Networks; New York City; November 29 - December 1, 1982
免疫网络会议;
  • 批准号:
    8208174
  • 财政年份:
    1982
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
Cellular Basis of Regulatory Immune Network
调节免疫网络的细胞基础
  • 批准号:
    8110578
  • 财政年份:
    1981
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant

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