Application of 15N Tracer Methodology and Flow Cytometry to the Study of Nitrogen Metabolism of Marine Phytoplankton

15N示踪方法和流式细胞术在海洋浮游植物氮代谢研究中的应用

• 批准号: 8711737
• 负责人: Steven Wofsy
• 金额: $ 12.43万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-11-01 至 1990-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8711737&HistoricalAwards=false
• 关键词: Application; 15N; Tracer; Methodology; Flow

This award supports a two year continuing grant for Dr. Wofsy and his co-investigator Dr. Lipschultz to begin work on nitrogen uptake by various subpopulations of marine particulate material. Previous measurements of nitrogen uptake by marine microbes has been done on bulk samples and this method has grouped the activity of many contributing subpopulations. The present investigators will separate the particulate populations using a cell sorter and analyze these small samples using newly developed modifications to emission band spectroscopy. This work will produce our first quantitative analysis of the influence of detrital material on nitrogen rate measurements that are thought to limit the biological productivity of much of the world ocean.

这项奖励支持为沃夫西博士和他的合作者利普舒尔茨博士提供的两年持续赠款，以开始研究海洋颗粒材料的不同亚群的氮吸收。以前对海洋微生物氮吸收的测量是在大样本上进行的，这种方法将许多贡献亚群的活动归类在一起。目前的研究人员将使用细胞分类器分离颗粒物种群，并使用新开发的对发射光谱的改进来分析这些小样本。这项工作将首次对碎屑物质对氮速率测量的影响进行量化分析，这些测量被认为限制了世界大部分海洋的生物生产力。