Macrocyst Genetics of Dictyostelium

该项目是为了优化发芽的条件， 盘基网柄藻的有性形成的大孢囊。 我们计划 到 1)筛选野生分离物，以寻找产生大孢囊的配对， 发芽速度快，速度快。 2)选择增加发芽的突变株。 3)用酶加速发芽， 大孢囊壁和信息素因子从其他 萌发的大孢囊 目标是达到至少10%的发芽率， 在休眠6周后， 适用于其他物种的常规使用。 遗传 将不会尝试使用巨囊系统的实验 在这个项目期间。 Dictyosteoprotein已被用于开发研究， 年数。 但它的潜力从未被实现 因为快速的遗传方法可以获得高频率的 不存在连锁群内的重组。 的 这个项目的成功完成将是伟大的 为科学界服务， 作为实验遗传学的网骨藻的发展 系统

The project is to optimize conditions for germination of the sexually formed macrocysts of Dictyostelium discoideum. We plan to 1) Screen wild isolates for pairs making macrocysts which germinate quickly and at a high rate. 2) Select mutant strains which increase germination. 3) Accelerate germination with enzymes which digest the macrocyst wall and with pheromonal factors from other germinating macrocysts. The goal is to achieve at least 10% germination of macrocysts after 6 weeks of dormancy, a rate which has proved adequate for routine use with other species. Genetic experiments which use macrocyst system will not be attempted during the period of this project. Dictyostelium has been used for studies in development for a number of years. But its potential has never been realized because rapid genetic methods for obtaining high-frequency recombination within a linkage group do not exist. The successful completion of this project would be of great service to the scientific community and a contribution to the development of Dictyostelium as an experimental genetic system.