Macrocyst Genetics of Dictyostelium

盘基网柄菌的大囊遗传学

• 批准号: 8719076
• 负责人: Robert Eisenberg
• 金额: $ 7.76万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1991-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8719076&HistoricalAwards=false
• 关键词: Macrocyst; Genetics; Dictyostelium

The project is to optimize conditions for germination of the sexually formed macrocysts of Dictyostelium discoideum. We plan to 1) Screen wild isolates for pairs making macrocysts which germinate quickly and at a high rate. 2) Select mutant strains which increase germination. 3) Accelerate germination with enzymes which digest the macrocyst wall and with pheromonal factors from other germinating macrocysts. The goal is to achieve at least 10% germination of macrocysts after 6 weeks of dormancy, a rate which has proved adequate for routine use with other species. Genetic experiments which use macrocyst system will not be attempted during the period of this project. Dictyostelium has been used for studies in development for a number of years. But its potential has never been realized because rapid genetic methods for obtaining high-frequency recombination within a linkage group do not exist. The successful completion of this project would be of great service to the scientific community and a contribution to the development of Dictyostelium as an experimental genetic system.

本研究的目的是优化盘状盘柱体（Dictyostelium disideum）有性形成大囊的萌发条件。我们的计划是1)筛选野生分离株，筛选出萌发速度快、萌发率高的大囊对。2)选择提高发芽率的突变株。3)利用消化大囊壁的酶和其他正在萌发的大囊的信息素因子来促进萌发。目标是在休眠6周后实现至少10%的大囊萌发，这一比率已被证明足以用于其他物种的常规使用。本项目期间将不尝试使用大囊系统进行遗传实验。盘基ostelium已被用于发展研究多年。但它的潜力从未被实现，因为在连锁群内获得高频重组的快速遗传方法不存在。该项目的成功完成将为科学界提供巨大的服务，并为盘基骨门作为一种实验遗传系统的发展做出贡献。