Sarcoplasmic Reticulum of a Fast Lobster Muscle

快速龙虾肌肉的肌浆网

• 批准号: 8903796
• 负责人: Robert Eisenberg
• 金额: $ 28.31万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-15 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8903796&HistoricalAwards=false
• 关键词: Sarcoplasmic; Reticulum; Fast; Lobster; Muscle

The sarcoplasmic reticulum (SR) is a flattened, convoluted sack within muscle cells that controls the movement of calcium ions, and thus regulates the contraction of muscle. It has been hard to study the properties of this membranous sack directly because it is so small and inaccessible within the muscle cell, but new techniques have removed both problems. Dr. Eisenberg has developed a mechanically skinned preparation of a specialized lobster muscle, the remotor of the second antenna, that contains a large amount of SR (70% of its volume) to control its very rapid contractions. This preparation can be studied with modern techniques of electrophysiology. Its membrane potential can be recorded directly, and the properties of the individual ion channels of its membrane can be measured. The objective of this research is a systematic study of the electrical properties of the SR compartment, emphasizing the properties likely to be involved in the control of the rapid contraction of the remotor muscle. The following features will be studied: the ions responsible for the resting potential of the SR and the effects of calcium ions, chloride ions, caffeine, and procaine which are known to affect excitation-contraction coupling. The two different types of calcium channel found in the SR will be studied, and agents that modulate their behavior will be sought. The resistance and capacitance of the SR compartment will be measured. This research is of great biological interest. Although it has been known for many years that the sarcoplasmic reticulum (SR) controls muscle contraction by regulating the levels of intracellular calcium, this will be the first time that direct measurements of the electrical properties of the SR membrane and its individual ion channels have been made in any preparation. This specialized, rapidly contracting lobster muscle should provide important clues to the general mechanisms of control of muscle contraction.

肌浆网（SR）是一个扁平、卷曲的囊状结构 在控制钙离子运动的肌肉细胞中， 从而调节肌肉的收缩。 这很难 直接研究这种膜囊的性质， 它是如此之小，无法进入肌肉细胞，但新的 技术解决了这两个问题。 艾森伯格博士 开发了一种专门的机械去皮制剂 龙虾肌肉，第二个天线的遥控器，包含 大量的SR（其体积的70%），以控制其非常 快速收缩。 这种制备方法可以用现代 电生理学技术。 它的膜电位可以是 直接记录，和单个离子的属性 可以测量其膜的通道。 的目的 研究是系统地研究电性能的 SR隔室，强调属性可能是 参与控制电机的快速收缩 肌肉. 将研究以下特征： 负责SR的静息电位， 钙离子，氯离子，咖啡因和普鲁卡因， 已知影响兴奋-收缩偶联。 两 在SR中发现的不同类型的钙通道将是 研究，并将寻求调节其行为的药物。 SR隔室的电阻和电容将为 测定了 这项研究具有重大的生物学意义。 虽然 多年来，人们一直知道肌浆网（SR） 控制肌肉收缩的方法是调节 细胞内钙，这将是第一次直接 测量SR膜的电性能，以及 它的各个离子通道可以用任何制剂制成。 这种专门的，快速收缩的龙虾肌肉应该 提供了重要线索的一般机制的控制 肌肉收缩。