Molecular Systematics of Ribosomal RNA Genes in the Tricholomataceae

杜克大学的Rytas Vilgalys博士将使用现代DNA分析方法获取担子菌家族Tricholomataceae三个相关部落真菌的核苷酸序列数据和限制性位点图谱信息。基因组数据将用于提高对这些真菌的属群的识别，并确定属和部落之间的系统发育亲缘关系。这些真菌将在核糖体RNA编码位点上进行系统发育上有用的限制性内切位点和序列变异调查。此外，Vilgalys博士还修改了丝状真菌的实验室程序，允许从粗细胞裂解物中分离的RNA进行快速、直接的测序。进一步的遗传分析将提供真菌核糖体基因进化速率和方向的信息。

Dr. Rytas Vilgalys of Duke University will use modern methods of DNA analysis to obtain nucleotide sequence data and restriction-site mapping information on fungi of three related tribes of the basidiomycete family Tricholomataceae. The genomic data will be used to improve recognition of generic groups of these fungi and to determine the phylogenetic affinities among the genera and tribes. The fungi will be surveyed for phylogenetically useful restriction site and sequence variation at loci coding for ribosomal RNA. In addition, Dr. Vilgalys has adapted laboratory procedures for filamentous fungi which allow rapid, direct sequencing of RNA from isolates from crude cellular lysates. Further genetic analyses will provide information about the rate and direction of evolution within ribosomal genes of fungi.