Cell - Surface Interactions in Biotechnology and Biomedicine

培养的依赖贴壁的哺乳动物细胞之间的相互作用 并将研究在其上培养它们的表面。 原代人内皮细胞和3T3细胞将在 表面的性质已被系统地改变， 小心控制。 衍生的甲基丙烯酸烷基酯的共聚物 将被用作培养表面，以确定表面的效果 自由能和化学部分如羧基，叔胺， 和季胺基团对细胞粘附、细胞形态和 细胞代谢 将在显微镜下测量细胞形态， 将包括最大附着力。 特定细胞的作用 还将研究粘附分子。 细胞代谢将是 通过测量细胞生长速率， 细胞外基质，生长因子的释放， 花生四烯酸代谢物的含量。 这些研究对应用细胞的发展具有重要意义 文化，用于生物技术和生物医学用途。 生物技术上，哺乳动物细胞培养用于生产关键的 和复杂的药物，以及他们的表现， 对生物反应器的表面特性知之甚少。 在生物医学上，需要涉及活细胞的组织替代物 附着在一些合成的支撑结构上，例如，血液 附着在合成管上作为人造血管的血管细胞 血管 然而，细胞功能的某些改变 表面相互作用的结果，这可以 包括组织替代的功能。

The interactions between cultured, anchorage-dependent mammalian cells and the surfaces upon which they are cultured will be investigated. Primary human endothelial cells and 3T3 cells will be cultured on surfaces whose properties have been systematically altered and carefully controlled. Copolymers of derivitized alkyl methacrylates will be used as the culture surface to determine the effect of surface free energy and chemical moieties such as carboxyl, tertiaryamine, and quaternary amine groups on cell adhesion, cell morphology, and cell metabolism. Cell morphology will be measured microscopically and will include the maximum force of adhesion. The role of specificcell adhesion molecules will also be investigated. Cell metabolism will be investigated by measuring the cell growth rate, the synthesis of extra cellular matrix, the release of growth factors, and there lease of arachidonic acid metabolites. These investigations are important to advances in applied cell culture, both for biotechnological and biomedical use. Biotechnologically, mammalian cell culture is used to produce critical and complex pharmaceuticals, and their performance with respect to the surface characteristics of the bioreactor is poorly understood. Biomedically, tissue replacements are desired which involve live cells attached to some synthetic support structure, for example, blood vessel cells attached to a synthetic tube to serve as an artificial blood vessel. Invariably, some alteration in cell function is observed as a result of the surface interactions, and this can comprise the function of the tissue replacement.