Cell - Surface Interactions in Biotechnology and Biomedicine

生物技术和生物医学中的细胞-表面相互作用

• 批准号: 8810268
• 负责人: Jeffrey Hubbell
• 金额: $ 10万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8810268&HistoricalAwards=false
• 关键词: Cell; Surface; Interactions; Biotechnology; Biomedicine

The interactions between cultured, anchorage-dependent mammalian cells and the surfaces upon which they are cultured will be investigated. Primary human endothelial cells and 3T3 cells will be cultured on surfaces whose properties have been systematically altered and carefully controlled. Copolymers of derivitized alkyl methacrylates will be used as the culture surface to determine the effect of surface free energy and chemical moieties such as carboxyl, tertiaryamine, and quaternary amine groups on cell adhesion, cell morphology, and cell metabolism. Cell morphology will be measured microscopically and will include the maximum force of adhesion. The role of specificcell adhesion molecules will also be investigated. Cell metabolism will be investigated by measuring the cell growth rate, the synthesis of extra cellular matrix, the release of growth factors, and there lease of arachidonic acid metabolites. These investigations are important to advances in applied cell culture, both for biotechnological and biomedical use. Biotechnologically, mammalian cell culture is used to produce critical and complex pharmaceuticals, and their performance with respect to the surface characteristics of the bioreactor is poorly understood. Biomedically, tissue replacements are desired which involve live cells attached to some synthetic support structure, for example, blood vessel cells attached to a synthetic tube to serve as an artificial blood vessel. Invariably, some alteration in cell function is observed as a result of the surface interactions, and this can comprise the function of the tissue replacement.

培养的依赖于锚定的哺乳动物细胞和它们被培养的表面之间的相互作用将被研究。原代人类内皮细胞和3T3细胞将在其特性经过系统改变和仔细控制的表面上培养。用衍生的甲基丙烯酸烷基酯共聚物作为培养表面，测定表面自由能和化学基团如羧基、叔胺和季胺基团对细胞黏附、细胞形态和细胞代谢的影响。细胞形态将在显微镜下测量，并将包括最大粘附力。特定的细胞黏附分子的作用也将被研究。通过测量细胞生长速率、细胞外基质的合成、生长因子的释放和花生四烯酸代谢产物的释放来研究细胞代谢。这些研究对应用细胞培养在生物技术和生物医学上的应用都是重要的。在生物技术方面，哺乳动物细胞培养被用来生产关键和复杂的药物，而它们在生物反应器表面特征方面的表现却鲜为人知。在生物医学上，需要将活细胞连接到一些合成支撑结构上的组织替换，例如，连接到合成管上的血管细胞作为人造血管。作为表面相互作用的结果，总是观察到细胞功能的一些变化，这可能包括组织替换的功能。