Acquisition of a Spectrofluorometer

技术的重大进步使监测成为可能 活细胞内离子活动的快速变化。 该技术首先在细胞中加入荧光染料， 对不同离子（Ca、H、Cl）具有特异性。 离子变化 然后，通过监测来自 将细胞保持在荧光分光光度计的比色皿内。 这种涉及细胞群的基本技术可以 扩展到单细胞分析， 硬件和显微镜。 数字化图像的重建， 一台微型计算机可以对细胞进行二维和三维分析， 离子浓度的变化。 目前，在赖特州立大学， 大学，有多达8名独立调查员 使用相关的荧光技术， 仪器 现有的仪器具有某些缺点， 以及被大量使用。 然而，足够重要的 已经获得的初步数据促使五名研究人员 申请仪器仪表计划下的资金， 另一种双光束激发荧光分光光度计，光电二极管 阵列检测、单细胞（显微镜）和成像 能力的 这五位研究者的研究方案 涉及钙，pH值和氯化物的测量， 细胞类型，包括星形胶质细胞，血管平滑肌， 人二倍体成纤维细胞。 此外，要求 荧光分光光度计将用于涉及膜的研究 融合，例如在胞吐或病毒感染期间发生的融合， 入侵

Major advances in technology have made it possible to monitor rapid changes in intracellular ion activities in living cells. the technique involves first loading cells with fluorescent dyes specific for different ions (Ca , H , Cl ). Changes in ion concentration are then measured by monitoring fluorescence from the cells maintained inside the cuvette of a spectrofluorometer. this basic technique involving a population of cells can be extended to analysis of single cells with the appropriate hardware and microscope. Reconstruction of a digitized image by a mini-computer allows 2 and 3-D analysis of cells undergoing changes in ion concentrations. Currently, at Wright State University, there are as many as eight independent investigators using related fluorescent techniques and sharing time on a single instrument. The available instrument has certain drawbacks as well as being heavily used. However, enough significant preliminary data has been obtained to prompt five researchers to request funds under the Instrumentation Program to purchase another spectrofluorometer with dual beam excitation, photodiode array detection, single cell (microscopic) and imaging capabilities. The research proposal of these five investigators involve the measurement of calcium, pH, and chloride in diverse cell types, including astrocytes, vascular smooth muscle, and human diploid fibroblasts. In addition, the requested spectrofluorometer will be used for studies involving membrane fusion such as that which occurs during exocytosis or viral invasion.